Title:
METHOD FOR SCREENING FOR S1P LYASE INHIBITOR USING CULTURED CELL
Document Type and Number:
WIPO Patent Application WO/2012/002274
Kind Code:
A1
Abstract:
Disclosed is a screening method using a cultured cell, which enables the rapidly, simply and highly sensitively finding of a compound that has an SPL inhibition activity and therefore can increase the amount of sphingosine 1-phosphate or dihydrosphingosine 1-phosphate by the SPL inhibition activity. Specifically disclosed are: a rapid and simple measurement method employing a scintillation proximity assay (SPA); a method for greatly improving the sensitivity of the detection of an activity of a compound by controlling the concentration of vitamin B6 in a culture medium that is used in assaying; and others.
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Inventors:
OTOYO MAMORU
TAMURA MASAKAZU
TAMURA MASAKAZU
Application Number:
PCT/JP2011/064535
Publication Date:
January 05, 2012
Filing Date:
June 24, 2011
Export Citation:
Assignee:
DAIICHI SANKYO CO LTD (JP)
OTOYO MAMORU
TAMURA MASAKAZU
OTOYO MAMORU
TAMURA MASAKAZU
International Classes:
C12Q1/02; C12Q1/68; G01N33/15; G01N33/50; G01N33/92
Domestic Patent References:
| WO2002027318A1 | 2002-04-04 |
Foreign References:
| JP2001518303A | 2001-10-16 | |||
| JP2005531285A | 2005-10-20 | |||
| JP2004509638A | 2004-04-02 |
Other References:
SERRA M. ET AL.: "Sphingosine 1-phosphate lyase, a key regulator of sphingosine 1-phosphate signaling and function.", ADV. ENZYME REGUL., vol. 50, no. 1, 13 November 2009 (2009-11-13), pages 349 - 362, XP027035225
SCHWAB S.R. ET AL.: "Lymphocyte sequestration through SIP lyase inhibition and disruption of SIP gradients.", SCIENCE, vol. 309, no. 5741, 9 September 2005 (2005-09-09), pages 1735 - 1739, XP002629304
LE STUNFF H. ET AL.: "Role of sphingosine- 1-phosphate phosphatase 1 in epidermal growth factor-induced chemotaxis.", J. BIOL. CHEM., vol. 279, no. 33, 2004, pages 34290 - 34297, XP055069067
REISS U. ET AL.: "Sphingosine-phosphate lyase enhances stress-induced ceramide generation and apoptosis.", J. BIOL. CHEM., vol. 279, no. 2, 2004, pages 1281 - 1290, XP055069068
KIM D.H. ET AL.: "Elevation of sphingoid base 1- phosphate as a potential contributor to hepatotoxicity in fumonisin B1-exposed mice.", ARCH. PHARM. RES., vol. 30, no. 8, 2007, pages 962 - 969, XP055069074
EXPERT OPIN. THER. TARGETS., vol. 13, no. 8, 2009, pages 1013 - 1025
PLOS ONE., vol. 4, no. 1, 2009, pages E4112
SCIENCE, vol. 309, no. 5741, 2005, pages 1735 - 1739
J. LIPID RES., vol. 48, no. 12, 2007, pages 2769 - 2778
BIOCHEM. BIOPHYS. RES. COMMUN., vol. 380, no. 2, 2009, pages 366 - 370
CHEMBIOCHEM., vol. 10, no. 5, 2009, pages 820 - 822
ANAL. BIOCHEM., vol. 230, 1995, pages 315 - 320
ANAL. BIOCHEM., vol. 272, 1999, pages 80 - 86
ANAL. BIOCHEM., vol. 282, 2000, pages 115 - 120
PROSTAGLANDINS & OTHER LIPID MEDIATORS, vol. 84, 2007, pages 154 - 162
PROC. NATL. ACAD. SCI. USA., vol. 103, no. 44, 2006, pages 16394 - 16399
See also references of EP 2586874A4
SCHWAB S.R. ET AL.: "Lymphocyte sequestration through SIP lyase inhibition and disruption of SIP gradients.", SCIENCE, vol. 309, no. 5741, 9 September 2005 (2005-09-09), pages 1735 - 1739, XP002629304
LE STUNFF H. ET AL.: "Role of sphingosine- 1-phosphate phosphatase 1 in epidermal growth factor-induced chemotaxis.", J. BIOL. CHEM., vol. 279, no. 33, 2004, pages 34290 - 34297, XP055069067
REISS U. ET AL.: "Sphingosine-phosphate lyase enhances stress-induced ceramide generation and apoptosis.", J. BIOL. CHEM., vol. 279, no. 2, 2004, pages 1281 - 1290, XP055069068
KIM D.H. ET AL.: "Elevation of sphingoid base 1- phosphate as a potential contributor to hepatotoxicity in fumonisin B1-exposed mice.", ARCH. PHARM. RES., vol. 30, no. 8, 2007, pages 962 - 969, XP055069074
EXPERT OPIN. THER. TARGETS., vol. 13, no. 8, 2009, pages 1013 - 1025
PLOS ONE., vol. 4, no. 1, 2009, pages E4112
SCIENCE, vol. 309, no. 5741, 2005, pages 1735 - 1739
J. LIPID RES., vol. 48, no. 12, 2007, pages 2769 - 2778
BIOCHEM. BIOPHYS. RES. COMMUN., vol. 380, no. 2, 2009, pages 366 - 370
CHEMBIOCHEM., vol. 10, no. 5, 2009, pages 820 - 822
ANAL. BIOCHEM., vol. 230, 1995, pages 315 - 320
ANAL. BIOCHEM., vol. 272, 1999, pages 80 - 86
ANAL. BIOCHEM., vol. 282, 2000, pages 115 - 120
PROSTAGLANDINS & OTHER LIPID MEDIATORS, vol. 84, 2007, pages 154 - 162
PROC. NATL. ACAD. SCI. USA., vol. 103, no. 44, 2006, pages 16394 - 16399
See also references of EP 2586874A4
Attorney, Agent or Firm:
ISHIBASHI Koki et al. (JP)
Kimiki Ishibashi (JP)
Kimiki Ishibashi (JP)
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