Title:
METHOD FOR SCREENING SUBSTANCES THAT AFFECT FORMATION, STRUCTURE, OR FUNCTION OF HUMAN BLOOD VESSELS, AND METHOD FOR PRODUCING HUMAN BLOOD VESSELS
Document Type and Number:
WIPO Patent Application WO/2019/230756
Kind Code:
A1
Abstract:
A method for screening substances that promote or suppress the formation of human blood vessels, or substances that normalize the structure or function of human blood vessels, the method including: (1) a step for cutting part of the skin of a non-human immunodeficient animal and exposing subcutaneous tissue or a muscle layer; (2) a step for placing human tissue on the exposed subcutaneous tissue or muscle layer; (3) a step for sealing the placed human tissue so that the placed human tissue is not in contact with the air; (4) a step for grafting the human tissue to the non-human immunodeficient animal; (5) a step for administering a substance to be tested into the non-human immunodeficient animal to which the human tissue was grafted as obtained in steps (1) to (4); (6) a step for observing the formation or structure of human blood vessels in the human blood tissue, and/or evaluating the function of the human blood vessels; and (7) a step for comparing with human blood vessels in a non-human immunodeficient animal to which human tissue was grafted and in which no substance to be tested was administered, and selecting either a substance to be tested that promotes or suppresses the formation of human blood vessels, or a substance to be tested that normalizes the structure or function of the human blood vessels.
Inventors:
TAKAKURA NOBUYUKI (JP)
TSUKADA YOHEI (JP)
TSUKADA YOHEI (JP)
Application Number:
PCT/JP2019/021215
Publication Date:
December 05, 2019
Filing Date:
May 29, 2019
Export Citation:
Assignee:
UNIV OSAKA (JP)
International Classes:
G01N33/50; A61L27/36; A61L27/50; C12Q1/02; G01N33/15; C12N5/071
Domestic Patent References:
| WO2011149013A1 | 2011-12-01 |
Foreign References:
| JP2015529660A | 2015-10-08 | |||
| US20040220390A1 | 2004-11-04 | |||
| JP2005521402A | 2005-07-21 |
Other References:
XINRU, X. ET AL.: "Study on the Antiangiogenic Potency and the Inhibition Effect of BA 11 on Tumor Growth", JOURNAL OF CAPITAL UNIVERSITY OF MEDICAL SCIENCE, vol. 27, no. 3, June 2006 (2006-06-01), pages 351 - 354
CHEN, Q. ET AL.: "Targeting tumor microvessels using doxorubicin encapsulated in a novel thermosensitive liposome", MOLECULAR CANCER THERAPEUTICS, vol. 3, no. 10, 2004, pages 1311 - 1317, XP055662542
RUPERTUS, K. ET AL.: "Rapamycin Inhibits Hepatectomy-Induced Stimulation of Metastatic Tumor Growth by Reduction of Angiogenesis, Microvascular Blood Perfusion, and Tumor Cell Proliferation", ANNALS OF SURGICAL ONCOLOGY, vol. 16, 2009, pages 2629 - 2637, XP019726958, DOI: 10.1245/s10434-009-0564-8
LEUNIG, M. ET AL.: "Angiogenesis, Microvascular Architecture, Microhemodynamics, and Interstitial Fluid Pressure during Early Growth of Human Adenocarcinoma LSI74T in SCID Mice", CANCER RESEARCH, vol. 52, December 1992 (1992-12-01), pages 6553 - 6560, XP055662546
HEUSER, M. ET AL.: "Exocrine contamination of isolated islets of Langerhans deteriorates the process of revascularization after free transplantation", TRANSPLANTATION, vol. 69, no. 5, 2000, pages 756 - 761
CHEN, Q. ET AL.: "Targeting tumor microvessels using doxorubicin encapsulated in a novel thermosensitive liposome", MOLECULAR CANCER THERAPEUTICS, vol. 3, no. 10, 2004, pages 1311 - 1317, XP055662542
RUPERTUS, K. ET AL.: "Rapamycin Inhibits Hepatectomy-Induced Stimulation of Metastatic Tumor Growth by Reduction of Angiogenesis, Microvascular Blood Perfusion, and Tumor Cell Proliferation", ANNALS OF SURGICAL ONCOLOGY, vol. 16, 2009, pages 2629 - 2637, XP019726958, DOI: 10.1245/s10434-009-0564-8
LEUNIG, M. ET AL.: "Angiogenesis, Microvascular Architecture, Microhemodynamics, and Interstitial Fluid Pressure during Early Growth of Human Adenocarcinoma LSI74T in SCID Mice", CANCER RESEARCH, vol. 52, December 1992 (1992-12-01), pages 6553 - 6560, XP055662546
HEUSER, M. ET AL.: "Exocrine contamination of isolated islets of Langerhans deteriorates the process of revascularization after free transplantation", TRANSPLANTATION, vol. 69, no. 5, 2000, pages 756 - 761
Attorney, Agent or Firm:
IWATANI, Ryo (JP)
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