Title:
METHOD FOR SCREENING αβ-TCR HETERODIMER MUTANTS
Document Type and Number:
WIPO Patent Application WO/2022/227066
Kind Code:
A1
Abstract:
A method for screening αβ-TCR mutants, the method comprising the construction of a phagemid. In the construction, a gene encoding an A1 polypeptide and a gene encoding a B1 polypeptide are inserted into the 3' ends of genes encoding the constant regions of the α chain and the β chain of TCR respectively, and a ribosome binding site and a molecular chaperone gene are integrated into a phage vector, wherein the amino acid sequence of the A1 polypeptide is as shown in SEQ ID NO: 1 or is represented by the variant of the A1 polypeptide; the amino acid sequence of the B1 polypeptide is as shown in SEQ ID NO: 2 or is represented by the variant of the B1 polypeptide; and the variants at least retain the function of the polypeptides before mutation. The A1 peptide and the B1 peptide are used for promoting the correct pairing and folding of the αβ heterodimer TCR, so that the efficiency of displaying the correctly folded TCR on the surface of a phage is improved. The method can efficiently complete the screening of αβ heterodimer TCR mutants.
Inventors:
WU XIANHUI (CN)
CHEN LIANG (CN)
TANG XIAOXIN (CN)
LIU XIAOBIAO (CN)
LI HONGJIAN (CN)
CHEN LIANG (CN)
TANG XIAOXIN (CN)
LIU XIAOBIAO (CN)
LI HONGJIAN (CN)
Application Number:
PCT/CN2021/091681
Publication Date:
November 03, 2022
Filing Date:
April 30, 2021
Export Citation:
Assignee:
SHENZHEN PREGENE BIOPHARMA CO LTD (CN)
International Classes:
C07K14/725; C07K19/00; C12N15/62
Domestic Patent References:
| WO2001000814A2 | 2001-01-04 |
Foreign References:
| CN112442118A | 2021-03-05 | |||
| US20140121123A1 | 2014-05-01 | |||
| CN110036034A | 2019-07-19 |
Other References:
ARNDT, K. M. ET AL.: "A Heterodimeric Coiled-coil Peptide Pair Selected in Vivo from a Designed Library-versus-Library Ensemble", JOURNAL OF MOLECULAR BIOLOGY, vol. 295, no. 3, 21 January 2000 (2000-01-21), pages 627 - 639, XP004461514, DOI: 10.1006/jmbi.1999.3352
BIRNBAUM, M. E. ET AL.: "Molecular architecture of the αβ T cell receptor–CD3 complex", PNAS, vol. 111, no. 49, 9 December 2014 (2014-12-09), pages 17576 - 17581, XP055619343, DOI: 10.1073/pnas.1420936111
NAT MED, vol. 18, 2012, pages 980
CLIN CANCER RES, vol. 26, 2020, pages 5869
ANNU REV IMMUNOL, vol. 21, 2003, pages 139
PROTEIN ENG DES SEL, vol. 24, 2011, pages 361
JBIOL CHEM, vol. 280, 2005, pages 1882
NAT COMMUN, vol. 10, 2019, pages 4451
NAT COMMUN, vol. 11, 2020, pages 2330
CELLS, vol. 9, 2020, pages 1485
METHODS MOL BIOL, vol. 1319, 2015, pages 95
J IMMUNOL METHODS, vol. 221, 1998, pages 59
NAT BIOTECHNOL, vol. 23, 2005, pages 349
J BIOL CHEM, vol. 294, 2019, pages 5790
BIOMOL ENG, vol. 24, 2007, pages 361
PROTEIN ENG, vol. 16, 2003, pages 707
NAT BIOTECH, vol. 23, 2005, pages 349
PROTEIN SCI, vol. 8, 1999, pages 2418
J IMMUNOL METHODS, vol. 236, 2000, pages 147
J IMMUNOL METHODS, vol. 306, 2005, pages 51
SCIENCE, vol. 274, 1996, pages 209
J. MOL. BIOL., vol. 285, 1999, pages 1831
PROTEIN ENG., vol. 16, 2003, pages 707
BIRNBAUM, M. E. ET AL.: "Molecular architecture of the αβ T cell receptor–CD3 complex", PNAS, vol. 111, no. 49, 9 December 2014 (2014-12-09), pages 17576 - 17581, XP055619343, DOI: 10.1073/pnas.1420936111
NAT MED, vol. 18, 2012, pages 980
CLIN CANCER RES, vol. 26, 2020, pages 5869
ANNU REV IMMUNOL, vol. 21, 2003, pages 139
PROTEIN ENG DES SEL, vol. 24, 2011, pages 361
JBIOL CHEM, vol. 280, 2005, pages 1882
NAT COMMUN, vol. 10, 2019, pages 4451
NAT COMMUN, vol. 11, 2020, pages 2330
CELLS, vol. 9, 2020, pages 1485
METHODS MOL BIOL, vol. 1319, 2015, pages 95
J IMMUNOL METHODS, vol. 221, 1998, pages 59
NAT BIOTECHNOL, vol. 23, 2005, pages 349
J BIOL CHEM, vol. 294, 2019, pages 5790
BIOMOL ENG, vol. 24, 2007, pages 361
PROTEIN ENG, vol. 16, 2003, pages 707
NAT BIOTECH, vol. 23, 2005, pages 349
PROTEIN SCI, vol. 8, 1999, pages 2418
J IMMUNOL METHODS, vol. 236, 2000, pages 147
J IMMUNOL METHODS, vol. 306, 2005, pages 51
SCIENCE, vol. 274, 1996, pages 209
J. MOL. BIOL., vol. 285, 1999, pages 1831
PROTEIN ENG., vol. 16, 2003, pages 707
Attorney, Agent or Firm:
SHANGHAI BESHINING LAW OFFICE (CN)
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