Title:
METHOD OF SELECTING CELLS
Document Type and Number:
WIPO Patent Application WO/2018/124189
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a method of selecting cell lines in which the reduction of a recombinant protein has been suppressed. The present invention pertains to a method of selecting cells, said method comprising a first step in which the intracellular expression level of at least one gene selected from among genes having any one of the base sequences represented by SEQ ID NO: 1-16 and orthologs thereof is measured, and a second step in which the expression level achieved by suppressing the reduction of a recombinant protein is evaluated on the basis of the difference determined by comparing the gene expression level measured in the first step and a control value for the gene expression level.
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Inventors:
YAMAMOTO KOICHI (JP)
MATSUMOTO YUICHI (JP)
YAMAGUCHI KEINA (JP)
FUJII HIROTO (JP)
SUZAWA TOSHIYUKI (JP)
MATSUMOTO YUICHI (JP)
YAMAGUCHI KEINA (JP)
FUJII HIROTO (JP)
SUZAWA TOSHIYUKI (JP)
Application Number:
PCT/JP2017/046939
Publication Date:
July 05, 2018
Filing Date:
December 27, 2017
Export Citation:
Assignee:
KYOWA HAKKO KIRIN CO LTD (JP)
International Classes:
C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12P21/00; C12P21/08; C12Q1/04; C12Q1/68
Domestic Patent References:
| WO2014054744A1 | 2014-04-10 |
Foreign References:
| US20060030022A1 | 2006-02-09 | |||
| JP2014129358A | 2014-07-10 | |||
| US6946292B2 | 2005-09-20 | |||
| JP2016256279A | 2016-12-28 |
Other References:
TAFT, S. ET AL.: "Toxicity of Anthrax Toxin Is Influenced by Receptor Expression", CLINICAL AND VACCINE IMMUNOLOGY, vol. 15, no. 9, 1 September 2008 (2008-09-01), pages 1330 - 1336, XP055605714, DOI: 10.1128/CVI.00103-08
DATABASE NBCI 27 May 2016 (2016-05-27), "Cricetulus griseus", XP003503622, retrieved from ncbi Database accession no. PRJNA72741
XU, X. ET AL.: "The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line", NATURE BIOTECHNOLOGY, vol. 29, no. 8, 31 July 2011 (2011-07-31), pages 735 - 741, XP055223996, DOI: 10.1038/nbt.1932
KAO, Y.H. ET AL.: "Mechanism of antibody reduction in cell culture production processes", BIOTECHNOLOGY AND BIOENGINEERING, vol. 107, no. 4, 13 September 2010 (2010-09-13), pages 622 - 632, XP002694207, DOI: 10.1002/bit.22848
TREXLER-SCHMIDT, M. ET AL: "Identification and prevention of antibody disulfide bond reduction during cell culture manufacturing", BIOTECHNOLOGY AND BIOENGINEERING, vol. 106, no. 3, 22 February 2010 (2010-02-22), pages 452 - 461, XP002694206, DOI: 10.1002/bit.22699
BIOTECHNOLOGY AND BIOENGINEERING, vol. 106, no. 3, 2010, pages 452 - 461
BIOENGINEERING AND BIOTECHNOLOGY, vol. 112, no. 4, 2015, pages 734 - 742
JOURNAL OF BIOTECHNOLOGY, vol. 261-267, 2012, pages 157
GENOME BIOL., vol. 3, no. 7, 2002, pages 1 - 11
YI QU ET AL., BMC BIOINFORMATICS, vol. 211, 2010, pages 11
See also references of EP 3564367A4
DATABASE NBCI 27 May 2016 (2016-05-27), "Cricetulus griseus", XP003503622, retrieved from ncbi Database accession no. PRJNA72741
XU, X. ET AL.: "The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line", NATURE BIOTECHNOLOGY, vol. 29, no. 8, 31 July 2011 (2011-07-31), pages 735 - 741, XP055223996, DOI: 10.1038/nbt.1932
KAO, Y.H. ET AL.: "Mechanism of antibody reduction in cell culture production processes", BIOTECHNOLOGY AND BIOENGINEERING, vol. 107, no. 4, 13 September 2010 (2010-09-13), pages 622 - 632, XP002694207, DOI: 10.1002/bit.22848
TREXLER-SCHMIDT, M. ET AL: "Identification and prevention of antibody disulfide bond reduction during cell culture manufacturing", BIOTECHNOLOGY AND BIOENGINEERING, vol. 106, no. 3, 22 February 2010 (2010-02-22), pages 452 - 461, XP002694206, DOI: 10.1002/bit.22699
BIOTECHNOLOGY AND BIOENGINEERING, vol. 106, no. 3, 2010, pages 452 - 461
BIOENGINEERING AND BIOTECHNOLOGY, vol. 112, no. 4, 2015, pages 734 - 742
JOURNAL OF BIOTECHNOLOGY, vol. 261-267, 2012, pages 157
GENOME BIOL., vol. 3, no. 7, 2002, pages 1 - 11
YI QU ET AL., BMC BIOINFORMATICS, vol. 211, 2010, pages 11
See also references of EP 3564367A4
Attorney, Agent or Firm:
Eikoh Patent Firm, P.C. (JP)
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