Title:
METHOD OF SELECTING GENOMIC DNA FRAGMENT
Document Type and Number:
WIPO Patent Application WO/2005/040374
Kind Code:
A1
Abstract:
It is intended to provide a method of selecting a genomic DNA fragment to be transferred into a plant for making an agriculturally advantageous improvement in the plant. Namely, a method comprising: (1) the step of preparing genomic DNA from a plant and constructing a genomic DNA library with the use of a cloning vector; (2) transferring individual genomic fragments contained in a plural number of genomic clones constituting the genomic DNA library into plants to give transformant plants; (3) culturing the transformant plants or offspring plants thereof and selecting a plant having an agriculturally advantageous mutation in the phenotype; and (4) selecting, as a desired genomic DNA fragment, a genomic DNA fragment having been transferred in the step (2) into the plant selected in the step (3).
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Inventors:
KUBO TOMOAKI (JP)
KOMARI TOSHIHIKO (JP)
USAMI SATORU (JP)
TAKAKURA YOSHIMITSU (JP)
HIEI YUKOH (JP)
ISHIDA YUJI (JP)
KOMARI TOSHIHIKO (JP)
USAMI SATORU (JP)
TAKAKURA YOSHIMITSU (JP)
HIEI YUKOH (JP)
ISHIDA YUJI (JP)
Application Number:
PCT/JP2004/015743
Publication Date:
May 06, 2005
Filing Date:
October 22, 2004
Export Citation:
Assignee:
JAPAN TOBACCO INC (JP)
KUBO TOMOAKI (JP)
KOMARI TOSHIHIKO (JP)
USAMI SATORU (JP)
TAKAKURA YOSHIMITSU (JP)
HIEI YUKOH (JP)
ISHIDA YUJI (JP)
KUBO TOMOAKI (JP)
KOMARI TOSHIHIKO (JP)
USAMI SATORU (JP)
TAKAKURA YOSHIMITSU (JP)
HIEI YUKOH (JP)
ISHIDA YUJI (JP)
International Classes:
A01H1/00; A01H1/04; A01H5/00; C12N5/14; C12N15/09; C12N15/82; C12Q1/68; (IPC1-7): C12N15/09; C12N5/14; A01H5/00; C12Q1/68
Foreign References:
| JPH10155485A | 1998-06-16 |
Other References:
OLSZEWSKI N.E. ET AL: "Specialized binary vector for plant transformation: expression of the Arabidopsis thaliana AHAS gene in Nicotiana tabacum", NUCLEIC ACIDS RES., vol. 16, no. 22, 25 November 1988 (1988-11-25), pages 10765 - 10782, XP000007464
KAWASAKI S. ET AL: "High capacity binary vector for complementation analysis of plant genome functions", BIO SCIENCE INDUSTRY, vol. 55, no. 7, 1997, pages 487 - 489, XP002997105
OTT R.W. ET AL: "Enhancer sequences from Arabidopsis thaliana obtained by library transformation of Nicotiana tabacum", MOL.GEN.GENET., vol. 223, no. 2, 1990, pages 169 - 179, XP001183104
SHI Z. ET AL: "Construction of a genomic library from a brown planthopper-resistant rice line using a transformation-competent vector and identification of clones spanning the Qbp1 locus", PLANT SCIENCE, vol. 165, October 2003 (2003-10-01), pages 879 - 885, XP002997106
HE R.F. ET AL: "Construction of a genomic library of wild rice and Agrobacterium-mediated transformation of large insert DNA linked to BPH resistance locus", GENE, vol. 321, 4 December 2003 (2003-12-04), pages 113 - 121, XP004474276
KAWASAKI S. ET AL: "High capacity binary vector for complementation analysis of plant genome functions", BIO SCIENCE INDUSTRY, vol. 55, no. 7, 1997, pages 487 - 489, XP002997105
OTT R.W. ET AL: "Enhancer sequences from Arabidopsis thaliana obtained by library transformation of Nicotiana tabacum", MOL.GEN.GENET., vol. 223, no. 2, 1990, pages 169 - 179, XP001183104
SHI Z. ET AL: "Construction of a genomic library from a brown planthopper-resistant rice line using a transformation-competent vector and identification of clones spanning the Qbp1 locus", PLANT SCIENCE, vol. 165, October 2003 (2003-10-01), pages 879 - 885, XP002997106
HE R.F. ET AL: "Construction of a genomic library of wild rice and Agrobacterium-mediated transformation of large insert DNA linked to BPH resistance locus", GENE, vol. 321, 4 December 2003 (2003-12-04), pages 113 - 121, XP004474276
Attorney, Agent or Firm:
Shamoto, Ichio (Section 206 New Ohtemachi Bldg., 2-1,
Ohtemachi 2-chome, Chiyoda-k, Tokyo 04, JP)
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