Title:
METHOD FOR SELECTING NUCLEIC ACIDS THAT BOND WITH HIGH-AFFINITY TO A TARGET
Document Type and Number:
WIPO Patent Application WO2002029093
Kind Code:
A3
Abstract:
The invention relates to a method for selecting nucleic acids that bond with high affinity to a target from a mixture of nucleic acids. The method comprises the following steps: a) loading the interior of a column with the target molecules and immobilising the target molecules in said column; b) feeding the mixture of nucleic acids into a first end of the column, to create a defined volumetric flow of medium through the column, running from the first end to the second end of said column; c) the nucleic acids bond with the target molecules with an affinity that decreases as the distance from the first end of the column increases and are immobilised; d) the volumetric flow of medium through the column is stopped after a defined period; e) the column is divided into column segments by a plurality of partitions, a routing co-ordinate being allocated to each segment; f) the immobilised nucleic acids from at least one segment are desorbed in a non-specific manner and are extracted using the allocation of the routing co-ordinate allocated to the segment.
Inventors:
KAGE ANDREAS (DE)
Application Number:
PCT/DE2001/003817
Publication Date:
May 01, 2003
Filing Date:
October 02, 2001
Export Citation:
Assignee:
KAGE ANDREAS (DE)
International Classes:
C12N15/09; C12Q1/68; C12Q1/6806; C12Q1/6811; C12Q1/6834; (IPC1-7): C12Q1/68; B01D15/08
Domestic Patent References:
| WO1991019813A1 | 1991-12-26 |
Foreign References:
| US5004547A | 1991-04-02 |
Other References:
FAMULOK M ET AL: "IN VITRO SELECTION OF SPECIFIC LIGAND-BINDING NUCLEIC ACIDS**", ANGEWANDTE CHEMIE. INTERNATIONAL EDITION, VERLAG CHEMIE. WEINHEIM, DE, vol. 31, no. 8, 1 August 1992 (1992-08-01), pages 979 - 988, XP000298700, ISSN: 0570-0833
PIERROU S ET AL: "SELECTION OF HIGH-AFFINITY BINDING SITES FOR SEQUENCE-SPECIFIC, DNABINDING PROTEINS FROM RANDOM SEQUENCE OLIGONUCLEOTIDES", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, SAN DIEGO, CA, US, vol. 229, no. 1, 20 July 1995 (1995-07-20), pages 99 - 105, XP000524716, ISSN: 0003-2697
THIESEN H J ET AL: "TARGET DETECTION ASSAY (TDA): A VERSATILE PROCEDURE TO DETERMINE DNA BINDING SITES AS DEMONSTRATED ON SP1 PROTEIN", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 18, no. 11, 11 June 1990 (1990-06-11), pages 3203 - 3209, XP000132496, ISSN: 0305-1048
PIERROU S ET AL: "SELECTION OF HIGH-AFFINITY BINDING SITES FOR SEQUENCE-SPECIFIC, DNABINDING PROTEINS FROM RANDOM SEQUENCE OLIGONUCLEOTIDES", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, SAN DIEGO, CA, US, vol. 229, no. 1, 20 July 1995 (1995-07-20), pages 99 - 105, XP000524716, ISSN: 0003-2697
THIESEN H J ET AL: "TARGET DETECTION ASSAY (TDA): A VERSATILE PROCEDURE TO DETERMINE DNA BINDING SITES AS DEMONSTRATED ON SP1 PROTEIN", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 18, no. 11, 11 June 1990 (1990-06-11), pages 3203 - 3209, XP000132496, ISSN: 0305-1048
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