Title:
METHOD FOR SELECTING PLURIPOTENT STEM CELL
Document Type and Number:
WIPO Patent Application WO/2013/186946
Kind Code:
A1
Abstract:
The present invention provides a novel method for evaluating/selecting a pluripotent stem cell with high efficiency and high accuracy while eliminating the occurrence of a cross reaction as observed in an antigen-antibody reaction. In the present invention, an N-binding sugar chain, an O-binding sugar chain, a GSL, a GAG, and a FOS on the surface of a cell are separated and purified from a single cell sample by a glycoblotting method or a BEP method, and the comprehensive quantitative/qualitative analysis of these sugar chains is carried out by MALDI-TOF/MS. In this manner, sugar chain markers specific to a pluripotent stem cell can be determined, and the sugar chain markers can be used for the identification of a pluripotent cell.
Inventors:
SHINOHARA YASURO (JP)
FURUKAWA JUN-ICHI (JP)
FUJITANI NAOKI (JP)
ARAKI KAYO (JP)
NAKAMURA YUKIO (JP)
FURUKAWA JUN-ICHI (JP)
FUJITANI NAOKI (JP)
ARAKI KAYO (JP)
NAKAMURA YUKIO (JP)
Application Number:
PCT/JP2012/078651
Publication Date:
December 19, 2013
Filing Date:
November 05, 2012
Export Citation:
Assignee:
UNIV HOKKAIDO NAT UNIV CORP (JP)
International Classes:
C12Q1/04; G01N27/62; G01N33/48; G01N33/53
Domestic Patent References:
| WO2010058605A1 | 2010-05-27 | |||
| WO2008093828A1 | 2008-08-07 |
Foreign References:
| JP2012037416A | 2012-02-23 |
Other References:
NORITAKA HASHII ET AL.: "Glycomics in Quality Control of Tissue-engineered Medical Products", JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN, vol. 132, no. 4, April 2012 (2012-04-01), pages 489 - 497, XP055104616
TERUHIDE YAMAGUCHI: "Saisei Iryo Jitsuyoka ni Muketa Saibo Soshiki Kako Iyakuhin no Anzensei Hinshitsu To no Kakuho ni Kansuru Kiban Gijutsu Kaihatsu Kenkyu", SAISEI IRYO JITSUYOKA NI MUKETA SAIBO SOSHIKI KAKO IYAKUHIN NO ANZENSEI HINSHITSU TO NO KAKUHO NI KANSURU KIBAN GIJUTSU KAIHATSU KENKYU HEISEI 22 NENDO SOKATSU BUNTAN KENKYU HOKOKUSHO, 2011, pages 1 - 93
TERUHIDE YAMAGUCHI: "Saisei Iryo Jitsuyoka ni Muketa Saibo Soshiki Kako Iyakuhin no Anzensei Hinshitsu To no Kakuho ni Kansuru Kiban Gijutsu Kaihatsu Kenkyu", SAISEI IRYO JITSUYOKA NI MUKETA SAIBO SOSHIKI KAKO IYAKUHIN NO ANZENSEI HINSHITSU TO NO KAKUHO NI KANSURU KIBAN GIJUTSU KAIHATSU KENKYU HEISEI 22 NENDO SOKATSU BUNTAN KENKYU HOKOKUSHO, 2011, pages 1 - 93
Attorney, Agent or Firm:
SAMEJIMA, Mutsumi et al. (JP)
Mutsumi Sameshima (JP)
Mutsumi Sameshima (JP)
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