Title:
METHOD FOR SELECTIVELY CULTURING MICROORGANISM USING PHOSPHITE DEHYDROGENASE GENE AS MARKER
Document Type and Number:
WIPO Patent Application WO/2014/024998
Kind Code:
A1
Abstract:
Provided is a method for selectively culturing a microorganism in a simple manner and at low cost, which enables the selective culture of a microorganism of interest without employing any sterilizing procedure or without using any antibiotic. In the method for selectively culturing a microorganism according to the present invention, a microorganism into which a phosphite dehydrogenase gene is introduced is cultured in a culture medium containing phosphorous acid as a sole phosphorous source.
Inventors:
KURODA AKIO (JP)
HIROTA RYUICHI (JP)
ISHIDA TAKENORI (JP)
HIROTA RYUICHI (JP)
ISHIDA TAKENORI (JP)
Application Number:
PCT/JP2013/071569
Publication Date:
February 13, 2014
Filing Date:
August 08, 2013
Export Citation:
Assignee:
UNIV HIROSHIMA (JP)
International Classes:
C12N1/21; C12N1/15; C12N1/19; C12N5/10; C12N15/09
Domestic Patent References:
| WO2010058298A2 | 2010-05-27 | |||
| WO2012147556A1 | 2012-11-01 | |||
| WO2010058298A2 | 2010-05-27 |
Other References:
METCALF, W. W. ET AL.: "Molecular Genetic Analysis of Phosphite and Hypophosphite Oxidation by Pseudomonas stutzeri WM88", J. BACTERIOL., vol. 180, no. 21, 1998, pages 5547 - 5558, XP002979982
HIROTA, R. ET AL.: "Isolation and characterization of a soluble and thermostable phosphite dehydrogenase from Ralstonia sp. strain 4506", J. BIOSCI. BIOENG., vol. 113, no. 4, April 2012 (2012-04-01), pages 445 - 450, XP055181193
WOODYER, R. ET AL.: "Relaxing the Nicotinamide Cofactor Specificity of Phosphite Dehydrogenase by Rational Design", BIOCHEMISTRY, vol. 42, 2003, pages 11604 - 11614, XP002311089
SATOSHI ONO ET AL.: "Arinsan Dehydrogenase Idenshi o Sentaku Marker to shita Biseibutsu Tairyo Baiyoho no Kaihatsu", JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY 2013 NENDO TAIKAI KOEN YOSHISHU, 5 March 2013 (2013-03-05), XP055184866
LOPEZ-ARREDONDO, D. L. ET AL.: "A novel dominant selectable system for the selection of transgenic plants under in vitro and greenhouse conditions based on phosphite metabolism", PLANT BIOTECHNOL. J., vol. 11, May 2013 (2013-05-01), pages 516 - 525, XP055181196
"Nikkei Baio Saishin Y6go Jiten", 17 September 2002, NIKKEI BUSINESS PUBLICATIONS, INC., pages: 846
WILLIAM W. METCALF; RALPH S. WOLFE, JOURNAL OF BACTERIOLOGY, vol. 180, no. 21, 1998, pages 5547 - 5558
HIROTA ET AL., J. BIOSCI. BIOENG., vol. 113, 2012, pages 445 - 450
SAMBROOK ET AL.: "Molecular Cloning, A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY
KATZBERG M. ET AL., INT. J. MOL. SCI., vol. 11, 2010, pages 1735 - 1758
WOODYER ET AL., BIOCHEMISTRY, vol. 42, 2003, pages 11604 - 11614
K. MOTOMURA ET AL., FEMS MICROBIOL. LETT., vol. 320, 2011, pages 25 - 32
WATANABE ET AL., MOL MICROBIOL., vol. 83, no. 4, February 2012 (2012-02-01), pages 856 - 65
BLOMFIELD IC ET AL., MOL. MICROBIOL., vol. 5, no. 6, June 1991 (1991-06-01), pages 1439 - 45
FORSBURG S.; RHIND N., YEAST, vol. 23, 2006, pages 173 - 183
MATSUYAMA A. ET AL., YEAST, vol. 21, 2004, pages 1289 - 1305
See also references of EP 2860242A4
HIROTA, R. ET AL.: "Isolation and characterization of a soluble and thermostable phosphite dehydrogenase from Ralstonia sp. strain 4506", J. BIOSCI. BIOENG., vol. 113, no. 4, April 2012 (2012-04-01), pages 445 - 450, XP055181193
WOODYER, R. ET AL.: "Relaxing the Nicotinamide Cofactor Specificity of Phosphite Dehydrogenase by Rational Design", BIOCHEMISTRY, vol. 42, 2003, pages 11604 - 11614, XP002311089
SATOSHI ONO ET AL.: "Arinsan Dehydrogenase Idenshi o Sentaku Marker to shita Biseibutsu Tairyo Baiyoho no Kaihatsu", JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY 2013 NENDO TAIKAI KOEN YOSHISHU, 5 March 2013 (2013-03-05), XP055184866
LOPEZ-ARREDONDO, D. L. ET AL.: "A novel dominant selectable system for the selection of transgenic plants under in vitro and greenhouse conditions based on phosphite metabolism", PLANT BIOTECHNOL. J., vol. 11, May 2013 (2013-05-01), pages 516 - 525, XP055181196
"Nikkei Baio Saishin Y6go Jiten", 17 September 2002, NIKKEI BUSINESS PUBLICATIONS, INC., pages: 846
WILLIAM W. METCALF; RALPH S. WOLFE, JOURNAL OF BACTERIOLOGY, vol. 180, no. 21, 1998, pages 5547 - 5558
HIROTA ET AL., J. BIOSCI. BIOENG., vol. 113, 2012, pages 445 - 450
SAMBROOK ET AL.: "Molecular Cloning, A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY
KATZBERG M. ET AL., INT. J. MOL. SCI., vol. 11, 2010, pages 1735 - 1758
WOODYER ET AL., BIOCHEMISTRY, vol. 42, 2003, pages 11604 - 11614
K. MOTOMURA ET AL., FEMS MICROBIOL. LETT., vol. 320, 2011, pages 25 - 32
WATANABE ET AL., MOL MICROBIOL., vol. 83, no. 4, February 2012 (2012-02-01), pages 856 - 65
BLOMFIELD IC ET AL., MOL. MICROBIOL., vol. 5, no. 6, June 1991 (1991-06-01), pages 1439 - 45
FORSBURG S.; RHIND N., YEAST, vol. 23, 2006, pages 173 - 183
MATSUYAMA A. ET AL., YEAST, vol. 21, 2004, pages 1289 - 1305
See also references of EP 2860242A4
Attorney, Agent or Firm:
HARAKENZO WORLD PATENT & TRADEMARK (JP)
Patent business corporation Hara [Kenzo] international patent firm (JP)
Patent business corporation Hara [Kenzo] international patent firm (JP)
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