Title:
METHOD FOR SENSING AND QUANTIFYING Ε-CAPROLACTAM BY USING REDESIGNED GENE CIRCUIT
Document Type and Number:
WIPO Patent Application WO/2015/072776
Kind Code:
A1
Abstract:
The present invention relates to a method for sensing and quantifying ε-caprolactam by using a redesigned gene circuit and, more particularly, to a method for analyzing and quantifying ε-caprolactam by using a gene circuit which can sense ε-caprolactam which is used as a precursor of nylon-6. The redesigned gene circuit recognizing ε-caprolactam according to the present invention allows a regulatory gene of ε-caprolactam to induce the expression of a fluorescent protein in proportion to an amount of ε-caprolactam. Thus, it is possible to rapidly sense and quantify ε-caprolactam. Therefore, the redesigned gene circuit can be used for the purpose of quantitatively analyzing ε-caprolactam and analyzing the activity of an enzyme so as to biosynthesize ε-caprolactam. Through the present invention, a trace amount - i.e., about 0.1 μm of ε-caprolactam - can be sensed by means of high-sensitivity, and analysis in cell units, which was not possible in the related art, is made possible. Thus, the present invention can be effectively used for a screening process of a novel gene and strain which produce ε-caprolactam from a microorganism metagenome library by using high-throughput screening.
Inventors:
LEE SEUNG GOO (KR)
YEOM SOO-JIN (KR)
KIM HASEONG (KR)
LEE DAE-HEE (KR)
KWON KIL KOANG (KR)
RHA EUGENE (KR)
JUNG HEUNG CHAE (KR)
YEOM SOO-JIN (KR)
KIM HASEONG (KR)
LEE DAE-HEE (KR)
KWON KIL KOANG (KR)
RHA EUGENE (KR)
JUNG HEUNG CHAE (KR)
Application Number:
PCT/KR2014/010951
Publication Date:
May 21, 2015
Filing Date:
November 14, 2014
Export Citation:
Assignee:
KOREA RES INST OF BIOSCIENCE (KR)
International Classes:
C12N15/11; C12N15/63; C12Q1/68
Foreign References:
| KR101222056B1 | 2013-01-14 | |||
| JPH10221325A | 1998-08-21 |
Other References:
HERAI, S. ET AL.: "Hyper-inducible expression system for streptomycetes", PROC. NATL. ACAD. SCI. USA, vol. 101, no. 39, 17 September 2004 (2004-09-17), pages 14031 - 14035
PANDEY, A. K. ET AL.: "Nitrile-inducible gene expression in mycobacteria", TUBERCULOSIS, vol. 89, no. 1, 17 September 2008 (2008-09-17), pages 12 - 16
BONIFACI, L. ET AL.: "Analysis of e -caprolactam and its cyclic oligomers by high-performance liquid chromatography", JOURNAL OF CHROMATOGRAPHY A, vol. 585, no. 2, 1 November 1991 (1991-11-01), pages 333 - 336
PANDEY, A. K. ET AL.: "Nitrile-inducible gene expression in mycobacteria", TUBERCULOSIS, vol. 89, no. 1, 17 September 2008 (2008-09-17), pages 12 - 16
BONIFACI, L. ET AL.: "Analysis of e -caprolactam and its cyclic oligomers by high-performance liquid chromatography", JOURNAL OF CHROMATOGRAPHY A, vol. 585, no. 2, 1 November 1991 (1991-11-01), pages 333 - 336
Attorney, Agent or Firm:
LEE, Cheo Young et al. (KR)
이처영 (KR)
이처영 (KR)
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