Title:
METHOD FOR SEPARATING ACTIVE INGREDIENTS FROM SIRAITIA GROSVENORII FLOWERS
Document Type and Number:
WIPO Patent Application WO/2022/247097
Kind Code:
A1
Abstract:
A method for separating active ingredients from Siraitia grosvenorii flowers, comprising: S1, extracting Siraitia grosvenorii flowers with water vapor to obtain wet flowers and a first extracting solution; S2, extracting the wet flowers with ethanol as a solvent at 25-65°C to obtain a second extracting solution and a first filter residue, concentrating the second extracting solution, and performing solid-liquid separation to obtain a third extracting solution and a wet residue; S3, concentrating the third extracting solution, and standing to obtain a crystal and a crystallization mother liquor; and S4, eluting a macroporous adsorption resin chromatographic column on the crystallization mother liquor sequentially with an acid solution, an alkali solution, and an alcohol solution to respectively obtain an acid eluent, an alkali eluent, and an alcohol eluent. According to the method, essential oil, flower dew, polysaccharide, Grosvenorine, alkaloid and triterpenoid saponin of Siraitia grosvenorii flowers can be obtained at the same time, and efficient comprehensive separation of Siraitia grosvenorii flowers is achieved.
Inventors:
ZHANG BAOTANG (CN)
TAN JIAZHONG (CN)
ZHENG SIJIA (CN)
JIANG LEWEN (CN)
FAN BIN (CN)
TAN JIAZHONG (CN)
ZHENG SIJIA (CN)
JIANG LEWEN (CN)
FAN BIN (CN)
Application Number:
PCT/CN2021/122932
Publication Date:
December 01, 2022
Filing Date:
October 09, 2021
Export Citation:
Assignee:
HUNAN NUTRAMAX INC (CN)
International Classes:
A61K36/42; C07C39/04; C07D311/36; C07G5/00; C08B37/00; C11B9/00; A61K133/00
Foreign References:
| CN113398157A | 2021-09-17 | |||
| CN113041279A | 2021-06-29 | |||
| CN107586820A | 2018-01-16 | |||
| CN105713061A | 2016-06-29 |
Other References:
BIAN WEI-MING, RONG-FEI LIU, FAN YANG, ZHANG ZHONG-SHAN: "Extraction and activity of flavone and polysaccharides from Siraitia grosvenorii flower", CEREALS & OILS, CN, vol. 33, no. 10, 10 October 2020 (2020-10-10), CN , pages 121 - 124, XP093007225, ISSN: 1008-9578
WANG HONG-TAO, KE XUE: "Optimization of extraction process of total polyphenols from Siraitia grosvenorii flower", CEREALS & OILS, CN, vol. 33, no. 8, 10 August 2020 (2020-08-10), CN , pages 77 - 80, XP093007230, ISSN: 1008-9578
LIANG ZHI-YUAN, GAN XIU-HAI; GAN ZHENG-YANG; ZHOU MEI: "Effects of volatile oil components from the Siraitia grosvenorii flowers by different extraction methods", LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH, SHIZHEN GUOYI GUOYAO ZAZHISHE, CN, vol. 25, no. 7, 20 July 2014 (2014-07-20), CN , pages 1602 - 1604, XP093007236, ISSN: 1008-0805, DOI: 10.3969/j.issn.1008-0805.2014.07.026
WANG HONG-TAO, KE XUE: "Optimization of extraction process of total polyphenols from Siraitia grosvenorii flower", CEREALS & OILS, CN, vol. 33, no. 8, 10 August 2020 (2020-08-10), CN , pages 77 - 80, XP093007230, ISSN: 1008-9578
LIANG ZHI-YUAN, GAN XIU-HAI; GAN ZHENG-YANG; ZHOU MEI: "Effects of volatile oil components from the Siraitia grosvenorii flowers by different extraction methods", LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH, SHIZHEN GUOYI GUOYAO ZAZHISHE, CN, vol. 25, no. 7, 20 July 2014 (2014-07-20), CN , pages 1602 - 1604, XP093007236, ISSN: 1008-0805, DOI: 10.3969/j.issn.1008-0805.2014.07.026
Attorney, Agent or Firm:
BEIJING JIUCHENG INTELLECTUAL PROPERTY AGENCY (SPECIAL ORDINARY PARTNERSHIP) (CN)
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