Title:
METHOD FOR SEPARATING CELL FROM BIOLOGICAL TISSUE
Document Type and Number:
WIPO Patent Application WO/2014/119334
Kind Code:
A1
Abstract:
Provided is a cell separation method, whereby a cell having a high biological activity can be efficiently and stably separated from a biological tissue, characterized by comprising using a degrading enzyme composition that is prepared by adding, to a definite amount of a neutral protease and/or a protease derived from Clostridium sp., an enzyme capable of degrading a major protein in the biological tissue, the amount of said degrading enzyme being determined depending on the composition of the major protein in the biological tissue. According to this method, the kind and amount of a protein-degrading enzyme to be used for isolating a cell from a biological tissue can be determined on the basis of the composition of a major protein in the biological tissue. Thus, the amount of the protein-degrading enzyme to be used can be controlled and a cell having a high biological activity can be efficiently separated.
More Like This:
| WO/2003/092471 | CELL-SEEDED EXTRACELLULAR MATRIX GRAFTS |
| JPH09121848 | CORNEA TISSUE CULTURE AND OCULAR STIMULATION TEST |
| WO/1995/025547 | DEVICES AND METHODS FOR IMPLANTING TRANSDUCED CELLS |
Inventors:
GOTO MASAFUMI (JP)
MURAYAMA KAZUTAKA (JP)
YAMAGATA YOUHEI (JP)
WATANABE KIMIKO (JP)
MURAYAMA KAZUTAKA (JP)
YAMAGATA YOUHEI (JP)
WATANABE KIMIKO (JP)
Application Number:
PCT/JP2014/000527
Publication Date:
August 07, 2014
Filing Date:
January 31, 2014
Export Citation:
Assignee:
UNIV TOHOKU (JP)
MEIJI SEIKA PHARMA CO LTD (JP)
UNIV TOKYO NAT UNIV CORP (JP)
MEIJI SEIKA PHARMA CO LTD (JP)
UNIV TOKYO NAT UNIV CORP (JP)
International Classes:
C12N5/071; C12N1/00; C12N5/07
Domestic Patent References:
| WO2012124338A1 | 2012-09-20 | |||
| WO2010058707A1 | 2010-05-27 | |||
| WO1996000283A1 | 1996-01-04 | |||
| WO1998024889A1 | 1998-06-11 |
Foreign References:
| JPH09508026A | 1997-08-19 |
Other References:
BRANDHORST H. ET AL.: "The Importance of Tryptic-like Activity in Purified Enzyme Blends for Efficient Islet Isolation", TRANSPLANTATION, vol. 87, no. 3, 15 February 2009 (2009-02-15), pages 370 - 375, XP055269693
INOUE ET AL., J CELL BIOL, vol. 97, 1983, pages 1524 - 1539
SJ HUGHES; P MCSHANE, TRANSPLANT PROCEEDINGS, vol. 37, 2005, pages 3444 - 34445
SJ HUGHES; A CLARK; P MCSHANE, TRANSPLANTATION, vol. 81, no. 3, 2006, pages 423 - 426
E LINETSKY ET AL., DIABETES, vol. 46, 1997, pages 1120 - 1123
D BRANDHORST ET AL., TRANSPLANTATION PROCEEDINGS, vol. 37, no. 8, 2005, pages 3450 - 3451
H BRANDHORST ET AL., TRANSPLANTATION, vol. 87, no. 3, 2009, pages 370 - 375
INOUE ET AL., J CELL BIOL, vol. 97, 1983, pages 1524 - 1539
SJ HUGHES; P MCSHANE, TRANSPLANT PROCEEDINGS, vol. 37, 2005, pages 3444 - 34445
SJ HUGHES; A CLARK; P MCSHANE, TRANSPLANTATION, vol. 81, no. 3, 2006, pages 423 - 426
E LINETSKY ET AL., DIABETES, vol. 46, 1997, pages 1120 - 1123
D BRANDHORST ET AL., TRANSPLANTATION PROCEEDINGS, vol. 37, no. 8, 2005, pages 3450 - 3451
H BRANDHORST ET AL., TRANSPLANTATION, vol. 87, no. 3, 2009, pages 370 - 375
Attorney, Agent or Firm:
OHNO, Seiji et al. (JP)
Seiji Ono (JP)
Seiji Ono (JP)
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