Title:
METHOD FOR SEPARATING MEGAKARYOCYTES FROM PLATELETS, AND PLATELET SEPARATION KIT
Document Type and Number:
WIPO Patent Application WO/2018/169060
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing: a method for efficiently separating megakaryocytes from platelets produced from the megakaryocytes; and a platelet separation kit for efficiently separating megakaryocytes from platelets produced from the megakaryocytes. The present invention provides a method for separating megakaryocytes from platelets, the method comprising an incorporation step for incorporating magnetic microparticles into at least megakaryocytes; a culture step for producing platelets by culturing the megakaryocytes on a culture medium before and/or after the incorporation step; a magnetic field application step for applying a magnetic field to the culture medium after the incorporation step and culture step; and a recovery step for recovering the culture medium after the magnetic field application step.
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Inventors:
TAKEI TOSHIKI (JP)
YAMADA TADANORI (JP)
HATA KENICHIRO (JP)
YAMADA TADANORI (JP)
HATA KENICHIRO (JP)
Application Number:
PCT/JP2018/010507
Publication Date:
September 20, 2018
Filing Date:
March 16, 2018
Export Citation:
Assignee:
FUJIFILM CORP (JP)
International Classes:
C12N5/078; C12M1/00
Domestic Patent References:
WO2014208100A1 | 2014-12-31 | |||
WO2009122747A1 | 2009-10-08 | |||
WO2004110139A1 | 2004-12-23 |
Foreign References:
JP2006006166A | 2006-01-12 | |||
JPH11335289A | 1999-12-07 | |||
JP2016190824A | 2016-11-10 | |||
JP2016193896A | 2016-11-17 | |||
JP2007089563A | 2007-04-12 | |||
JPH06133784A | 1994-05-17 | |||
JP2008228728A | 2008-10-02 | |||
JP2003093499A | 2003-04-02 | |||
JP2006006166A | 2006-01-12 |
Other References:
BRAYLAN, RAUL ET AL.: "Rapid and Simple Procedure For Isolation and Concentration Of Human Megakaryocytes From Marrow Aspirates", BLOOD, vol. 122, no. 21, 2013, pages 5269, XP009516153, ISSN: 0006-4971
See also references of EP 3597732A4
See also references of EP 3597732A4
Attorney, Agent or Firm:
SIKS & CO. (JP)
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