Title:
METHOD FOR SITE-DIRECTED INTEGRATION OF TXGP1 GENE INTO RAJI CELL AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2019/237376
Kind Code:
A1
Abstract:
Disclosed is a method for the site-directed integration of the TXGP1 gene into a Raji cell, which method uses an insect protein expression system to synthesize the necessary components required for a recombinant adeno-associated virus (rAAV), and achieves the aim of integrating the TXGP1 gene in a site-directed manner into the AAVS1 locus of chromosome 19 in a Raji cell.
Inventors:
MAO JIYAN (CN)
Application Number:
PCT/CN2018/091706
Publication Date:
December 19, 2019
Filing Date:
June 16, 2018
Export Citation:
Assignee:
SHENZHEN BIOCAN TECH CO LTD (CN)
International Classes:
C12N15/12; C12N5/22; C12N15/86
Foreign References:
| CN102686732A | 2012-09-19 | |||
| CN104136613A | 2014-11-05 |
Other References:
XIA, YULONG: "Production of Recombinant Adeno-Associated Virus for Gene Therapy by Insect Cell Expression System", BASIC SCIENCES, CHINA MASTER'S THESES FULL-TEXT DATABASE, 15 December 2013 (2013-12-15), ISSN: 1674-0246
CHEN, YANING ET AL.: "Bac-to-Bacffl (Production of Adeno-Associated Virus Mediated by Bac-To-Bac Baculovirus Insect Expression System", CHINESE JOURNAL OF EXPERIMENTAL SURGERY, vol. 29, no. 7, 31 July 2012 (2012-07-31), pages 1363 - 1366, ISSN: 1001-9030
CHEN, YANING ET AL.: "Bac-to-Bacffl (Production of Adeno-Associated Virus Mediated by Bac-To-Bac Baculovirus Insect Expression System", CHINESE JOURNAL OF EXPERIMENTAL SURGERY, vol. 29, no. 7, 31 July 2012 (2012-07-31), pages 1363 - 1366, ISSN: 1001-9030
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