Title:
METHOD FOR SIZING POLYNUCLEOTIDES USING ELECTROPHORESIS WITH NON-DNA SIZE STANDARDS
Document Type and Number:
WIPO Patent Application WO2004034027
Kind Code:
A3
Abstract:
The present invention relates to a method of determining a size of a sample polynucleotide. A sample polynucleotide is subjected to electrophoresis in the presence of at least: a fluorescent compound having a first fluorescence spectrum, wherein detection of light of the first fluorescence spectrum is indicative of the presence of the sample polynucleotide, and a plurality of size standards, each of the size standards having a different mobility and wherein each of the size standards is essentially free of polynucleotides. Migration coordinates of the sample polynucleotide and plurality of size standards are determined. A size of the sample polynucleotide is determined using the migration coordinate of the sample polynucleotide and the migration coordinates of the size standards.
Inventors:
LIU ZHAOWEI (US)
KANE THOMAS (US)
KANE THOMAS (US)
Application Number:
PCT/US2003/032146
Publication Date:
November 11, 2004
Filing Date:
October 10, 2003
Export Citation:
Assignee:
SPECTRUMEDIX LLC (US)
LIU ZHAOWEI (US)
KANE THOMAS (US)
LIU ZHAOWEI (US)
KANE THOMAS (US)
International Classes:
C12Q1/68; (IPC1-7): G01N21/00; C07H21/04; C12Q1/68
Foreign References:
| US5599664A | 1997-02-04 | |||
| US5162514A | 1992-11-10 | |||
| US5302510A | 1994-04-12 |
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