Title:
METHOD FOR SPECIFICALLY KNOCKING OUT HUMAN MER6 GENE BY MEANS OF CRISPR-CAS9, AND SPECIFIC SGRNA THEREOF
Document Type and Number:
WIPO Patent Application WO/2019/237365
Kind Code:
A1
Abstract:
Provided are a method for specifically knocking out a human MER6 gene by means of CRISPR-Cas9, and a specific sgRNA thereof. By means of computer simulation, computation, and design, a group of sgRNAs specifically knocking out a specifically targeted MER6 gene in the human MER6 gene by using the CRISPR-Cas9 is synthesized, the sgRNA and a linear PX330 plasmid are separately connected to be a vector, the cell is transfected, i.e., the knockout of the MER6 gene can be realized. The sgRNA has the advantage that mass production can be performed with a small amount of synthesized polynucleotide fragments; a preparation method is simple and easy to perform; the sgRNA has good targetability; and the knockout efficiency of a CRISPR-Cas9 system is high.
Inventors:
MAO JIYAN (CN)
Application Number:
PCT/CN2018/091678
Publication Date:
December 19, 2019
Filing Date:
June 15, 2018
Export Citation:
Assignee:
SHENZHEN BIOCAN TECH CO LTD (CN)
International Classes:
C12N15/11; C12N15/85
Domestic Patent References:
| WO2016057980A1 | 2016-04-14 |
Foreign References:
| CN103820441A | 2014-05-28 | |||
| CN106222177A | 2016-12-14 |
Other References:
WEISKOPF, K. ET AL.: "CD 47-Blocking Immunotherapies Stimulate Macrophage-Mediated Destruction of Small- cell Lung Cancer", THE JOURNAL OF CLINICAL INVESTIGATION, vol. 126, no. 7, 31 July 2016 (2016-07-31), pages 2610 - 2620, XP055480649, DOI: 10.1172/JCI81603
YANG, YUN ET AL.: "The Role of CD 47 in Anti-tumor Immune Response", CHINESE JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 34, no. 3, 31 March 2018 (2018-03-31), pages 262 - 266
ZHAO, YA ET AL.: "Construction of Severe Combined Immunodeficiency Mice Based on CRSIPR/Cas9 Technology", ACTA LABORATORIUM ANIMALIS SCIENTIA SINICA, vol. 24, no. 4, 31 August 2016 (2016-08-31), pages 339 - 343
YANG, YUN ET AL.: "The Role of CD 47 in Anti-tumor Immune Response", CHINESE JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 34, no. 3, 31 March 2018 (2018-03-31), pages 262 - 266
ZHAO, YA ET AL.: "Construction of Severe Combined Immunodeficiency Mice Based on CRSIPR/Cas9 Technology", ACTA LABORATORIUM ANIMALIS SCIENTIA SINICA, vol. 24, no. 4, 31 August 2016 (2016-08-31), pages 339 - 343
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