Title:
A METHOD OF STRAIN DEVELOPMENT
Document Type and Number:
WIPO Patent Application WO/2020/145930
Kind Code:
A3
Abstract:
The invention is related to a method of development and selection of baker's yeast strains that can exhibit high raising activity in sweet and/or lean dough in the bakery and pastry industries, the method comprises the steps of; (i) developing the baker's yeast strain, preferably with the process of multiple crossing, by using the combination of one or more than one of the mutagenesis, evolutionary engineering, asexual hybridization, recombinant DNA technology and sexual hybridization methods; (ii) preparing the synthetic or flour-based selective nutrient medium, preferably the synthetic nutrient medium having a sugar content of 0%, 15% and/or 25%, and incubating the strains developed in multiple well plates inside the nutrient medium prepared; (iii) scanning the strains incubated by using a high-resolution optical scanner, or by the naked eye or a device with a spectrophotometric reader, preferably by using a high-resolution optical scanner, and selecting the strains that perform the best acidification which is to be determined based on the results of the analysis.
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Inventors:
SELIMOĞLU SÜREYYA MERT (TR)
ALEMDAR FILIZ (TR)
TÜRKER MUSTAFA (TR)
ALEMDAR FILIZ (TR)
TÜRKER MUSTAFA (TR)
Application Number:
PCT/TR2020/050016
Publication Date:
August 27, 2020
Filing Date:
January 10, 2020
Export Citation:
Assignee:
PAK GIDA ÜRETİM VE PAZARLAMA ANONİM ŞİRKETİ (TR)
International Classes:
A21D8/04; C12N1/18
Foreign References:
| US5399492A | 1995-03-21 | |||
| CA2012090C | 1997-03-25 | |||
| US10015972B2 | 2018-07-10 | |||
| US5741695A | 1998-04-21 |
Other References:
MARESOVA, LYDIE ET AL.: "Applications of a microplate reader in yeast physiology research", BIOTECHNIQUES, vol. 43, 2007, pages 667 - 72, XP055733162
Attorney, Agent or Firm:
DESTEK PATENT, INC. (TR)
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