Title:
METHOD FOR SUPPRESSING DIFFERENTIATION OF PLURIPOTENT STEM CELLS
Document Type and Number:
WIPO Patent Application WO/2021/162090
Kind Code:
A1
Abstract:
In the present invention, pluripotent stem cells are suspension cultured while being maintained in an undifferentiated state. During suspension culturing of the pluripotent stem cells, an undifferentiated state is maintained by adding a tankyrase inhibitor (TNKS inhibitor) and a PKCβ inhibitor from among PKC inhibitors.
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Inventors:
TAKEUCHI KAZUHIRO (JP)
IBUKI MASATO (JP)
HAYASHI YOHEI (JP)
TAKASAKI MAMI (JP)
KAMBAYASHI SHO (JP)
KAWAI YOSHIKAZU (JP)
IBUKI MASATO (JP)
HAYASHI YOHEI (JP)
TAKASAKI MAMI (JP)
KAMBAYASHI SHO (JP)
KAWAI YOSHIKAZU (JP)
Application Number:
PCT/JP2021/005255
Publication Date:
August 19, 2021
Filing Date:
February 12, 2021
Export Citation:
Assignee:
KANEKA CORP (JP)
RIKEN (JP)
RIKEN (JP)
International Classes:
C12N5/0735; C12N5/10; C12N9/99
Domestic Patent References:
| WO2019131940A1 | 2019-07-04 | |||
| WO2013077423A1 | 2013-05-30 | |||
| WO2019151386A1 | 2019-08-08 | |||
| WO2021020267A1 | 2021-02-04 |
Foreign References:
| US20130236961A1 | 2013-09-12 | |||
| JP2017525351A | 2017-09-07 | |||
| JP2020021843A | 2020-02-06 |
Other References:
ALEJANDRO DE LOS ANGELES: "Generation of ERK-Independent Human and Non-Human Primate Pluripotent Stem Cells", CURRENT PROTOCOLS IN STEM CELL BIOLOGY, vol. 49, no. e85, 30 November 2018 (2018-11-30), pages 1 - 17, XP009530434, ISSN: 1938-8969, DOI: 10.1002/cpsc.85
GE GUO, FERDINAND VON MEYENN, MARIA ROSTOVSKAYA, JAMES CLARKE, SABINE DIETMANN, DUNCAN BAKER, ANNA SAHAKYAN, SAMUEL MYERS, PAUL BE: "Epigenetic resetting of human pluripotency", DEVELOPMENT, THE COMPANY OF BIOLOGISTS LTD., GB, vol. 144, no. 15, 31 August 2017 (2017-08-31), GB, pages 2748 - 2763, XP055456607, ISSN: 0950-1991, DOI: 10.1242/dev.146811
OLMER R. ET AL., TISSUE ENGINEERING: PART C, vol. 18, no. 10, 2012, pages 772 - 784
M. KINEHARA ET AL., PLOS ONE, vol. 8, no. 5, 2013, pages e63378
SHAMBLOTT M. J. ET AL., PROC. NATL. ACAD. SCI. USA., vol. 95, 1998, pages 13726 - 13731
CONRAD S., NATURE, vol. 456, 2008, pages 344 - 349
YU J ET AL., SCIENCE, vol. 318, 2007, pages 1917 - 20
TAKAHASHI K ET AL., CELL, vol. 131, 2007, pages 861 - 72
See also references of EP 4105319A4
GE GUO, FERDINAND VON MEYENN, MARIA ROSTOVSKAYA, JAMES CLARKE, SABINE DIETMANN, DUNCAN BAKER, ANNA SAHAKYAN, SAMUEL MYERS, PAUL BE: "Epigenetic resetting of human pluripotency", DEVELOPMENT, THE COMPANY OF BIOLOGISTS LTD., GB, vol. 144, no. 15, 31 August 2017 (2017-08-31), GB, pages 2748 - 2763, XP055456607, ISSN: 0950-1991, DOI: 10.1242/dev.146811
OLMER R. ET AL., TISSUE ENGINEERING: PART C, vol. 18, no. 10, 2012, pages 772 - 784
M. KINEHARA ET AL., PLOS ONE, vol. 8, no. 5, 2013, pages e63378
SHAMBLOTT M. J. ET AL., PROC. NATL. ACAD. SCI. USA., vol. 95, 1998, pages 13726 - 13731
CONRAD S., NATURE, vol. 456, 2008, pages 344 - 349
YU J ET AL., SCIENCE, vol. 318, 2007, pages 1917 - 20
TAKAHASHI K ET AL., CELL, vol. 131, 2007, pages 861 - 72
See also references of EP 4105319A4
Attorney, Agent or Firm:
HIRAKI & ASSOCIATES (JP)
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