Title:
METHOD FOR SUPPRESSING TERATOMA FORMATION VIA SELECTIVE CELL DEATH INDUCTION IN UNDIFFERENTIATED HUMAN-INDUCED PLURIPOTENT STEM CELLS
Document Type and Number:
WIPO Patent Application WO/2014/058274
Kind Code:
A1
Abstract:
The present invention relates to a production method for induced-pluripotent-stem-cell-derived differentiated cells, from which undifferentiated induced pluripotent stem cells (iPS) have been eliminated, the method comprising the steps of: (a) preparing a cell sample comprising undifferentiated induced pluripotent stem cells and differentiated cells by differentiating induced pluripotent stem cells; and (b) selectively causing the death of the undifferentiated induced pluripotent stem cells by subjecting the product of step (a) to processing either with quercetin of formula 1 below or with YM-155 of formula 2 below. According to the present invention, the present invention makes it possible to effectively selectively cause the death only of undifferentiated induced pluripotent stem cells by causing induced pluripotent stem cells to differentiate into specific differentiated cells and then carrying out culturing in a differentiating culture medium comprising quercetin or YM-155, and, in the induced pluripotent stem cell differentiation method according to the present invention, only undifferentiated induced pluripotent stem cells that are a cause of teratoma formation are selectively caused to die, and thus differentiated differentiating cells are completely unaffected. In other words, the invention can be expected to ensure safety as the possibility of tumour formation during clinical use as a cell therapeutic agent is eliminated since the survival and functioning of the differentiated cells is maintained unchanged.
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Inventors:
CHA HYUK JIN (KR)
Application Number:
PCT/KR2013/009111
Publication Date:
April 17, 2014
Filing Date:
October 11, 2013
Export Citation:
Assignee:
UNIV SOGANG IND UNIV COOP FOUN (KR)
International Classes:
C12N5/074; C07D311/30; C12N1/36
Foreign References:
| KR20110042426A | 2011-04-27 | |||
| KR100717724B1 | 2007-05-14 |
Other References:
SHEN, S-C ET AL.: "Differential Apoptosis-Inducing Effect of Quercetin and its Glycosides in Human Promyeloleukemic HL-60 Cells by Alternative Activation of the Caspase 3 Cascade.", JOURNAL OF CELLULAR BIOCHEMISTRY, vol. 89, no. 5, 1 August 2003 (2003-08-01), pages 1044 - 1055
SASAKI, M. ET AL.: "Quercetin-Induced PC12 Cell Death Accompanied by Caspase-Mediated DNA Fragmentation.", BIOL. PHARM. BULL., vol. 30, no. 4, April 2007 (2007-04-01), pages 682 - 686
YAMANAKA, K. ET AL.: "Antitumor Activity ofYM155, a Selective Small-Molecule Survivin Suppressant, Alone and in Combination with Docetaxel in Human Malignant Melanoma Models.", CLINICAL CANCER RESEARCH, vol. 17, 7 July 2011 (2011-07-07), pages 5423 - 5431
SASAKI, M. ET AL.: "Quercetin-Induced PC12 Cell Death Accompanied by Caspase-Mediated DNA Fragmentation.", BIOL. PHARM. BULL., vol. 30, no. 4, April 2007 (2007-04-01), pages 682 - 686
YAMANAKA, K. ET AL.: "Antitumor Activity ofYM155, a Selective Small-Molecule Survivin Suppressant, Alone and in Combination with Docetaxel in Human Malignant Melanoma Models.", CLINICAL CANCER RESEARCH, vol. 17, 7 July 2011 (2011-07-07), pages 5423 - 5431
Attorney, Agent or Firm:
YANG, Boo-Hyun (1659-2 Inhundong Gwanak-gu, Seoul 151-832, KR)
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