Title:
METHOD FOR SURVEYING ECOSYSTEM IN WATER ENVIRONMENT BY USING ENVIRONMENTAL RNA
Document Type and Number:
WIPO Patent Application WO/2021/145384
Kind Code:
A1
Abstract:
Provided is a simple and highly accurate method for surveying an ecosystem while applying less burden on the environment. A method for analyzing biological species living in a water environment. In this method, RNA included in a water environment is analyzed to quantitatively assess and comprehensively identify the biological species living in the water environment.
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Inventors:
MIYATA KAEDE (JP)
HONDA HIROSHI (JP)
YAMANE MASAYUKI (JP)
HONDA HIROSHI (JP)
YAMANE MASAYUKI (JP)
Application Number:
PCT/JP2021/001076
Publication Date:
July 22, 2021
Filing Date:
January 14, 2021
Export Citation:
Assignee:
KAO CORP (JP)
International Classes:
C12Q1/68; A01K61/00; C12N15/09; C12Q1/6869
Foreign References:
| JP2017099376A | 2017-06-08 |
Other References:
NAKAMICHI TOMOKI, KENJI TSURI, YUICHIRO TERAZONO, KOTARO KAMO, TAKASHI ISHIHARA, HIROKI YAMANAKA: "P1-481 Comparison of the sensitivities of environmental DNA and RNA as markers for species detection from water samples and their time dependencies", 66TH ANNUAL MEETING OF THE ECOLOGICAL SOCIETY OF JAPAN; KOBE, JAPAN; MARCH 15-19, 2019, vol. 66, 2019, pages P1, XP009538350
JIANG, MING-YANG; MAEGAWA, KAZUYA; NAKAO, RYOHEI; MINAMOTO, TOSHIFUMI: "P2-074 Detection of common carp using eRNA from water samples", 66TH ANNUAL MEETING OF THE ECOLOGICAL SOCIETY OF JAPAN; KOBE, JAPAN; MARCH 15-19, 2019, vol. 66, 6 June 2019 (2019-06-06) - 19 March 2019 (2019-03-19), pages 1 - 1, XP009538351
MASAYUKI USHIO, HIROAKI MURAKAMI, REIJI MASUDA, TETSUYA SADO, MASAKI MIYA, SHO SAKURAI, HIROKI YAMANAKA, TOSHIFUMI MINAMOTO, MICHI: "Quantitative monitoring of multispecies fish environmental DNA using high-throughput sequencing", METABARCODING AND METAGENOMICS, vol. 2, 14 March 2018 (2018-03-14), pages e23297, XP055842475
MIYA M., SATO Y., FUKUNAGA T., SADO T., POULSEN J. Y., SATO K., MINAMOTO T., YAMAMOTO S., YAMANAKA H., ARAKI H., KONDOH M., IWASAK: "MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical marine species", ROYAL SOCIETY OPEN SCIENCE, vol. 2, no. 7, 1 July 2015 (2015-07-01), pages 150088, XP055842457
MIYA ET AL., ROYAL SOCIETY OPEN SCIENCE, vol. 2, no. 7, 2015, pages 150088
USHIO ET AL., METABARCODING AND METAGENOMICS, vol. 2, 2018, pages 1 - 15
NAKAMICHI ET AL., 66TH ANNUAL MEETING OF THE ECOLOGICAL SOCIETY OF JAPAN, 20 February 2019 (2019-02-20)
IJICHI ET AL., FIRST MEETING OF THE EDNA SOCIETY, TOKYO, 18 September 2018 (2018-09-18), pages 28
See also references of EP 4092131A4
JIANG, MING-YANG; MAEGAWA, KAZUYA; NAKAO, RYOHEI; MINAMOTO, TOSHIFUMI: "P2-074 Detection of common carp using eRNA from water samples", 66TH ANNUAL MEETING OF THE ECOLOGICAL SOCIETY OF JAPAN; KOBE, JAPAN; MARCH 15-19, 2019, vol. 66, 6 June 2019 (2019-06-06) - 19 March 2019 (2019-03-19), pages 1 - 1, XP009538351
MASAYUKI USHIO, HIROAKI MURAKAMI, REIJI MASUDA, TETSUYA SADO, MASAKI MIYA, SHO SAKURAI, HIROKI YAMANAKA, TOSHIFUMI MINAMOTO, MICHI: "Quantitative monitoring of multispecies fish environmental DNA using high-throughput sequencing", METABARCODING AND METAGENOMICS, vol. 2, 14 March 2018 (2018-03-14), pages e23297, XP055842475
MIYA M., SATO Y., FUKUNAGA T., SADO T., POULSEN J. Y., SATO K., MINAMOTO T., YAMAMOTO S., YAMANAKA H., ARAKI H., KONDOH M., IWASAK: "MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical marine species", ROYAL SOCIETY OPEN SCIENCE, vol. 2, no. 7, 1 July 2015 (2015-07-01), pages 150088, XP055842457
MIYA ET AL., ROYAL SOCIETY OPEN SCIENCE, vol. 2, no. 7, 2015, pages 150088
USHIO ET AL., METABARCODING AND METAGENOMICS, vol. 2, 2018, pages 1 - 15
NAKAMICHI ET AL., 66TH ANNUAL MEETING OF THE ECOLOGICAL SOCIETY OF JAPAN, 20 February 2019 (2019-02-20)
IJICHI ET AL., FIRST MEETING OF THE EDNA SOCIETY, TOKYO, 18 September 2018 (2018-09-18), pages 28
See also references of EP 4092131A4
Attorney, Agent or Firm:
THE PATENT CORPORATE BODY ARUGA PATENT OFFICE (JP)
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