R01DK-49108 awarded by the National Institutes of Health (NIH). The United States Government has certain rights in and to the invention described and claimed herein.

Field of the Invention The invention relates to a method for preparing the chelator, rhizoferrin.

Description of the Prior Art Iron is essential for almost all forms of life.

However, because of the aqueous insolubility of Fe (OH) 3, (Ksp = 2 x 10-39), the predominant form of the transition metal in the environment, virtually all life forms have developed rather sophisticated iron chelating and transport systems to utilize the metal. Higher animals tend to utilize proteins to transport and assimilate iron.

Microorganisms produce a group of low molecular weight chelators or siderophores [Bergeron,"Synthesis and Solution Structures of Microbial Siderophores,"Chem. Rev., Vol. 84, pages 587-602 (1984) ; Tait,"The Identification and Biosynthesis of Siderochromes Formed by Micrococcus denitrifi- cans, Biochem. J., Vol. 146, pages 191-204 (1975); Griffiths

et al,"Vibriobactin, a Siderophore from Vibrio cholerae,"J.

Biol. Chem., Vol. 259, pages 383-385 (1984); Aksoy et al, "Hypertransfusion and Iron Chelation in Thalassaemia,"page 80, Hans Huber Publishers, Berne (1985); and Bickel et al, "Metabolic products of actinomycetes. Ferrioxamine B,"Helv.

Chim. Acta., Vol. 43, pages 2129-2138 (1960)] for the purpose of acquiring iron. The metal exists in the biosphere largely in the insoluble ferric state and would be otherwise inacces- sible to bacteria without such ligands. Although a large number of siderophores have been identified, they fall largely into two structural classes: the catecholamides and the hydroxamates [Bergeron, supral. Many of the ligands of both structural types contain polyamine backbones. While the hexa- coordinate catecholamides parabactin [Tait, supra] and vibrio- bactin [Griffiths et al, supra] are predicated on the tri- amines spermidine and norspermidine, respectively, the hydrox- amates are frequently derived from the diamines, putrescine or cadaverine, or from their biochemical precursors, ornithine or lysine [Bergeron, supra]. For example, the siderophores iso- lated from Streptomyces pilosus, desferrioxamines A-I, consist of a group of hydroxamates with either repeating putrescine or cadaverine units in their backbones [Aksoy et al, supra]. The most well known of these chelators, desferrioxamine B (DFO) [Bickel et al, supra], is a linear trihydroxamate ligand which forms a very stable hexacoordinate, octahedral complex [Modell et al,"The Clinical Approach to Thalassaemia,"page 217, Grune and Stratton, London (1984)] with iron (III), Kf = 1 x

103° M-1. Although DFO binds a number of different +3 cations, e. g., A1 (III), Ga (III), Cr (III), it exhibits a high speci- ficity for iron (III). It is not too surprising then that the mesylate salt of desferrioxamine, DesferalQD, has been employed in the treatment of several iron overload diseases such as thalassemia [Anderson,"Inorganic Chemistry in Biology and Medicine,"Chapter 15, American Chemical Society, Washington, D. C. (1973); and Fisher et al,"Development of an Intravenous Desferrioxamine Mesylate Treatment Protocol for Swine: Moni- toring of Desferrioxamine and Metabolites By High-Performance Liquid Chromatography,"Pharmacology, Vol. 41, pages 263-271 (1990)]. However, the fact that patients must be continuously infused because of the short half-life of the drug in the body has compelled investigators to continue the search for better therapeutic iron chelators.

Nl Bis (l-oxo-3-hydroxy-3, 4-dicarboxybutyl) diamino- butane (rhizoferrin) was first isolated from Rhizopus micro- sporus var. rhizopodiformis, an organism associated with mucormycosis seen in dialysis patients [Drechsel et al, Biol.

Met., Vol. 4, pages 238-243 (1991)], and occurs in several Zygomycetes strains of fungi [Thieken et al, FEMS Microbiol.

Lett., Vol. 94, pages 37-42 (1992)]. Like the natural chela- tors parabactin and DFO, rhizoferrin forms a 1: 1 complex with ferric ion [Drechsel et al, Biol. Met., Vol. 5, pages 141-148 (1992)]; however, the formation constant of the chelate has not been measured. Structure determination of rhizoferrin [Drechsel et al, 1991, supra] revealed a putrescine center symmetrically diacylated by citric acid at its 1-carboxylate:

Thus, although rhizoferrin contains a polyamine backbone, it is not a member of either class of chelators.

Rather, it is a hydroxy polycarboxylate, along with rhizo- bactin [Smith, Tetrahedron Lett., Vol. 30, pages 313-316 (1989)] and staphyloferrin A [Konetschny-Rapp et al, Eur. J.

Biochem., Vol.! 91, pages 65-74 (1990)], which are predicated on L-lysine and D-ornithine, respectively. Unlike the hydrox- amates aerobactin, arthrobactin, schizokinen [Bergeron et al, "Synthesis of Catecholamide and Hydroxamate Siderophores,"in Handbook of Microbial Iron Chelators, Winkelmann, ed., CRC Press, Inc., Boca Raton, FL, pages 271-307 (1991)] and nanno- chelin [Samejima et al, Chem. Pharm. Bull., Vol. 32, pages 3428-3435 (1984)], in which citric acid is symmetrically 1,3-disubstituted, the prochiral carbon of each unsymmetric- ally functionalized citric acid in rhizoferrin is asymmetric.

These two sites of the molecule are in the (R)-configuration according to circular dichroism (CD) spectroscopy in compari- son with natural (R, R)--tartaric acid [Drechsel et al, 1992, supra].

There is a need for a method for synthesizing rhizo- ferrin and other compounds containing a citrate moiety of desired configuration in an amide linkage. The principal challenge to such a synthesis is to access a citrate synthon of correct configuration for coupling to an amine group in order to unequivocally define the absolute configuration of the final product.

It is an object of the present invention to provide such a synthetic route.

It is another object of the invention to provide novel heavy metal chelators and pharmaceutical compositions and methods for the use thereof.

SUMMARY OF THE INVENTION These and other objects are realized by the present invention, one embodiment of which relates to a method for synthesizing a compound of the formula: wherein : C* is a chiral carbon atom ; a and b may be the same or different and are inte- gers from 0 to 10, inclusive; and

R is H, alkyl, arylalkyl, carboxyl or wherein C* and b have the meanings ascribed above, comprising : (1) acylating a polyamine of the formula: wherein: a, b and R have the meanings ascribed above, and Q is an amine protective group, with a diester of citric acid having the formula: wherein: R'is alkyl, aryl, aralkyl or cyclo- alkyl having up to 10 carbon atoms, to produce an amide having the formula:

(2) hydrolyzing the amide (IV) to produce an acid having the formula: (3) deprotecting the acid (V) to remove the Q groups, thereby producing the acid of formula (I).

Another embodiment of the invention relates to certain novel heavy metal chelators having the formula : wherein: a, b, C* and R have the meanings ascribed above and R"is R'or Q, and salts thereof with pharmaceutically acceptable acids and cations.

An additional embodiment of the invention relates to pharmaceutical compositions in unit dosage form comprising a therapeutically effective amount of a compound of formula VI or a salt thereof with a pharmaceutically acceptable acid or cation and a pharmaceutically acceptable carrier therefor.

A further embodiment of the invention relates to methods for the treatment of human and non-human mammals in need thereof comprising the administration thereto of a thera- peutically effective amount of a compound of formula VI or a

salt thereof with a pharmaceutically acceptable acid or cation.

DETAILED DESCRIPTION OF THE INVENTION The invention will be described in detail with reference to the synthesis of rhizoferrin ; it being understood by those skilled in the art that the principles of the inven- tion as broadly described herein are applicable to the preparation of any compound embodying a citric acid moiety of particular enantiomeric configuration coupled via an amide linkage to an amine.

The synthetic scheme for preparing rhizoferrin is as follows: QQROOCOH HN-(CH2) 4-NH+Cl (II)@ \ ROOC-CHZ CH2-COOH (III) ROOCOH C* Q Q ROOC-CH2CH2CO-N- (CH2) 4-N-OC-CH2 2 C* > hydrotysis OHCOOR (IV) HOOCOH \ou C* Q Q HOOC-CH2CHZCO-N- (CHz) 4-N-OC-CH2 CH2COOH \/deprotect C* > HO COOH (V) HOOCOH C* HOOC-CH2CHzCONH- (CH ) 4-NH-OC-C 2 CHzCOOH C* HOCOOH (I) Theenantiomer(III)maybeaccessedviathe followingscheme: ROOCOH C*saponificationROOCOH > ROOC-CH2 CH2COORC* ROOC-CH2CH2COOH (VI) (VII) ROOC< C* ROOC-CH2CH2COOH (III)

In the above structural formulae and schemes, R may be the residue of any suitable esterifying alcohol having up to 10 carbon atoms such as alkyl (e. g., methyl, ethyl, propyl, butyl); aryl (e. g., phenyl); aralkyl (e. g., benzyl) or cycloalkyl (e. g., cyclopentyl, cyclobenzyl).

The diamine reactant may be any suitable amine con- taining primary amine groups such as those of formula (II).

Therein R may be alkyl, aryl, aralkyl or cycl. oalkyl, each having up to 10 carbon atoms or The expression"amino protective group" (Q) as used herein is intended to designate the Q group which is inserted in place of a hydrogen atom of an amino group or groups in order to protect the amino group (s) during synthesis.

Selection of a suitable amino protecting group will depend upon the reason for protection and the ultimate use of the protected product. When the protecting group is used solely for protection during synthesis, then a conventional amino protecting group may be employed. Appropriate amino protecting groups are known in the art and are described, for example, by Bodanszky in Principles of Synthesis, Springer- Verlag, New York (1984); by Ives in U. S. Patent No. 4,619,915; and in the various publications referred to in the latter.

See also Methoden der Orqanischen Chemie, Houben-Weyl, Vol.

15, No. 1, for protecting groups and Vol. 15, No. 2, for

methods of peptide synthesis. Representative amino protecting groups for synthetic use include benzyl and acyl groups such as tert-butoxycarbonyl, benzyloxycarbonyl, benzoyl, acetyl and the like. Yet other conventional amino protecting groups for use in synthesis are described in the literature [Bodanszky, supra, and Ives, supra].

The synthesis of rhizoferrin typically begins with trimethyl citrate which is converted to 1,2-dimethyl citrate by a sterically controlled saponification [Hirota et al, Chem- istry Lett., pages 191-194 (1980)]. The enantiomers of the carboxylic acid are separated by forming their (-)-brucine salts. After five fractional crystallizations from water, the crystalline salt is shown by single crystal X-ray diffraction to contain 1, 2-dimethyl citrate in the (R)-configuration.

Treatment of the salt with 1 N HC1 and extraction with ethyl acetate furnishes (R)-1, 2-dimethyl citrate.

With the correct enantiomeric acid in hand, N1, N4- dibenzyl-1, 4-diaminobutane [Samejima et al, supra] was acylated with (R)-1,2-dimethyl citrate (2 equivalents) utilizing diphenylphosphoryl azide (Et3N/DMF) [Shioiri et al, J. Am. Chem. Soc., Vol. 94, pages 6203-6205 (1972)]. The diamide was obtained in 26% yield after flash column chroma- tography, which removed by-products including olefins due to elimination of the tertiary alcohol as indicated by NMR. The methyl esters were hydrolyzed with sodium hydroxide in aqueous methanol and acidification gave N, N'-dibenzyl rhizoferrin.

Finally, since N-benzyl amides are resistant to hydrogenolysis [Williams et al, Tetrahedron Lett., Vol. 30, pages 451-454 (1989)], deprotection of the tetraacid under dissolving metal reduction conditions (Li/NH3/THF) [Kim et al, J. Orcf. Chem., Vol. 46, pages 5383-5389 (1981)], protonation of the salts on a cation exchange resin column and purifica- tion on a C-18 reversed-phase column furnished the final product, rhizoferrin. The high field NMR and high resolution mass spectrum of the synthetic compound were essentially identical to the published spectra of the natural product [Drechsel et al, 1991, supra]. The absolute configurations (R, R) of the synthetic sample and the natural material are identical since both exhibited a negative Cotton effect at the same wavelength [Drechsel et al, 1992, supra].

Rhizoferrin cyclizes upon standing through dehydra- tion to imidorhizoferrin and bis-imidorhizoferrin which possess one and two five-membered rings, respectively [Drechsel et al, 1992, supra]. It was observed by NMR that the zero order rate constant for this ring formation at pH 5.0 is 6.9 x 10-2 h-. At pH 3, the findings on the extent of cyclization were similar to the literature [Drechsel et al, 1992, supra) ; thus the analytical data were obtained before this decomposition occurred.

This synthetic methodology for rhizoferrin may also be used to prepare the hydroxy polycarboxylated siderophore staphyloferrin A [Konetschny-Rapp et al, supra ; and Meiwes et al, FEMS Microbiol. Lett., Vol. 67, pages 201-206 (1990)], in

which o-ornithine is N «, N-diacylated with citric acid at its 1-carboxylate. In addition, analogues of rhizoferrin in which the chain length of the central methylene bridge is varied can be synthesized for structure-activity studies.

The compounds of formula VI, useful as heavy metal chelators, are prepared in the same manner as those of formula V.

The pharmaceutical compositions of the invention preferably contain a pharmaceutically acceptable carrier suit- able for rendering the compound or mixture administrable orally as a tablet, capsule or pill, or parenterally or trans- dermally. The active ingredients may be admixed or compounded with any conventional, pharmaceutically acceptable carrier.

It will be understood by those skilled in the art that any mode of administration, vehicle or carrier conventionally employed and which is inert with respect to the active agent may be utilized for preparing and administering the pharmaceu- tical compositions of the present invention. Illustrative of such methods, vehicles and carriers are those described, for example, in Remington's Pharmaceutical Science, ad.

(1970), the disclosure of which is incorporated herein by reference. Those skilled in the art, having been exposed to the principles of the invention, will experience no difficulty in determining suitable and appropriate vehicles, excipients and carriers or in compounding the active ingredients there- with to form the pharmaceutical compositions of the invention.

The therapeutically effective amount of active agent to be included in the pharmaceutical composition of the inven- tion depends, in each case, upon several factors, e. g., the type, size and condition of the patient, the disorder to be treated, the intended mode of administration, the capacity of the patient to incorporate the intended dosage form, etc.

Generally, an amount of active agent is included in each dosage form to provide from about 50 to about 500 mg, prefer- ably from about 50 to about 250 mg.

The active agent employed in the pharmaceutical compositions and methods of treatment of the invention may comprise a pharmaceutically acceptable salt or complex of the compounds of formula I or II, e. g., sodium, potassium or other non-toxic metal salts, amine salts, etc., as well as acid salts with, e. g., HC1, HAc, etc.

The compound, compositions and method of the invention are useful for the treatment of heavy metal, e. g., iron, overload diseases such as thalassemia.

Those skilled in the art will be aware that the amounts of the various components of the compositions of the invention to be administered in accordance with the method of the invention to a patient will depend upon those factors noted above. Generally, however, amounts of active agent are administered to provide dosages thereof from about 50 to about 500 mg/kg, preferably from about 50 to about 250 mg/kg, the frequency of administration and duration of treatment being

dependent upon the type and nature of the patient and disorder being treated.

The invention is illustrated by the following non- limiting examples, wherein silica gel 32-63 (40 ßm'ßflash") or silica gel 60 (70-230 mesh) was used for column chroma- tography. Optical rotations were run in CH30H at 589 nm (Na lamp) at room temperature with c as grams of compound per 100 ml. 1H NMR spectra were recorded at 300 or 600 MHz and run in the deuterated organic solvent indicated or in D2O with chemi- cal shifts given in parts per million downfield from tetra- methylsilane or 3- (trimethylsilyl) propionic-2,2,3, acid, sodium salt, respectively. X-ray diffraction data were collected at 173K on a Siemens SMART PLATFORM equipped with a CCD area detector and a graphite monochromator utilizing Moka radiation (A. = 0.71073 A). Cell parameters were refined using up to 6233 reflections. A hemisphere of data (1381 frames) was collected using the-scan method (0. width). The first 50 frames were remeasured at the end of data collection to monitor instrument and crystal stability (maximum correc- tion on I was < 1%). Psi scan absorption corrections were applied based on the entire data set.

Circular dichroism spectra were obtained with a Jasco Model J500C spectropolarimeter equipped with a Jasco IF-500II interface and CompuAdd 286 computer; data collection and processing were performed with Jasco DP-500/PC System ver- sion 1.28 software. The cell path length was 2.00 cm.

Ultraviolet spectroscopy spectra were obtained with a Shimadzu UV-2501PC equipped with an AST 486/33 computer data station. The cell path length was 1.00 cm.

EXAMPLE 1 1,2-Dimethyl citrate (2) was prepared by modifica- tion of a published method [Hirota et al, supra]. Sodium hydroxide (0.1 N, 215 ml) was added to a solution of trimethyl citrate (1) (10.0 g, 42.7 mmol) in 50% aqueous (CH30H (200 ml) over 2 hours with vigorous stirring at room temperature. The solution was concentrated to about 150 ml and extracted with EtOAc (3 x 150 ml). The aqueous layer was acidified with 1 N HC1 (45 ml) and extracted with EtOAc (3 x 150 ml). The organic layer was dried (MgSO4) and concentrated, providing 3.70 g (39%) of (2) was a colorless oil : 1H NMR (d6-DMSO) 6 5.60 (br s, 1 H, OH), 3.64 (s, 3 H, CO2CH3), 3.57 (s, 3 H, CO2CH3), 2.87 (d, 1 H, J = 15 Hz, 1/2 C_ 2) 2.81 (d, 1 H, = 15 Hz, 1/2 Ci2), 2.65 (d, 1 H, J = 15 Hz, 1/2 C_2).

EXAMPLE 2 1, 2-Dimethyl-3- [ (S)-sec-phenethyl] citrate (3) was prepared by adding 1,3-dicyclohexylcarbodiimide (103 mg, 0. 5 mmol) to a solution of (2) (110 mg, 0.5 mmol), (S)- (-)-sec- phenethyl alcohol (61 mg, 0.5 mmol) and 4-dimethylamino- pyridine (3 mg) in dry CH2Cl2 (10 ml) at 0°C, and the mixture was stirred overnight. The mixture was filtered and the fil- trate was concentrated and purified by flash chromatography

(1: 2 EtOAc/hexane), resulting in 60 mg (37%) of (3) as a colorless oil : 1H NMR (CDC13) S 7.35-7.28 (m, Ph), 5.97 (q, J = 7 Hz, CHPh), 5.88 (q, J = 7 Hz, CHPh), 3.77 (s, CH30), 3.73 (s, C_3O), 3.69 (s, CH30), 3.68 (s, CH30), 2.98-2.74 (m, C_2), 1 ! 54 (d, J = 7 Hz, C-CH3), 1.52 (d, J = 7 Hz, C-CH3).

EXAMPLE 3 (-)-Brucine salt of (R)-1, 2-dimethyl citrate was prepared by adding (2) (7 g, 31. 8 mmol) to a solution of (-)-brucine (12.5 g, 31.8 mmol) (CAUTION: toxic) in EtOAc (460 ml) with vigorous stirring overnight. After filtration, the precipitate (10.5 g) was recrystallized from water (5x) and dried to afford 2.04 g of white crystals: mp 165-168°C.

The diastereomeric salt crystallizes in the mono- clinic space group C2 and has cell dimensions: a = 13.8947 (3), b = 12.4224 (3), and c = 17.5408 (3) A ; a = 90°, B = 104.556 (1), and 6 = 90°. The structure was solved by the Direct Methods in SHELXTL [Sheldrick, SHELXTL, Siemens XRD Corporation, Madison, WI (1995)] and was refined using full matrix least squares. The non-H atoms were treated aniso- tropically. The methyl hydrogen atoms were calculated in ideal positions and were riding on their respective carbon atoms; the rest of the H atoms were refined without con- straints. Two water molecules were located in the asymmetric unit. One was refined with full occupancy and its H atoms were located. The other water molecule, located on a 2-fold axis of rotation, was refined to a 30% occupancy. An absolute

configuration of (R) was assigned to the citrate portion of the salt based on knowledge of the stereochemistry of brucine.

Parameters (521) were refined in the final cycle of refinement using 3855 reflections with I > 2 a (I) to yield Ri and wR2 of 0.0434 and 0.1040, respectively. Refinement was conducted using F2.

EXAMPLE 4 (R)-1, 2-Dimethyl citrate (4). HC1 (1 N, 4 ml) was added to a solution of the (-)-brucine salt of (R)-1, 2- dimethyl citrate (2.04 g, 3.32 mmol) in water (50 ml) and stirring was continued for 5 minutes. Extraction with EtOAc (3 x 50 ml), drying over Na2SO4 and concentration gave 630 mg (86%) of (4) as a colorless oil: [a] +4.0 (c 1.00); the NMR was identical to (2).

EXAMPLE 5 N, N'-Dibenzyl rhizoferrin, tetraethyl ester (6).

Diphenylphosphoryl azide (760 mg, 2.76 mmol) and NEt3 (1.5 ml, 11 mmol) were added to a solution of (4) (610 mg, 2.77 mmol) and N1, N4-dibenzyl-1, 4-diaminobutane [Samejima et al, supra] (370 mg, 1.38 mmol) in DMF (20 ml) at 0°C under nitrogen. The solution was stirred at 0°C for 1 hour and then at room temperature for 23 hours. After solvents were removed under high vacuum, the residue was taken up in EtOAc (25 ml) and was washed with saturated NaHCO3 (25 ml), water (25 ml), 0.5 N HC1 (25 ml) and water (25 ml). The organic layer was dried

(MgSO4) and concentrated. Flash chromatography, eluting with 4: 1 EtOAc/hexane, generated 240 mg (26%) of (6) as a pale yellow oil: [a] +8.25 (c 1.00) ; 1H NMR (CDC13) 6 7.42-7.24 (m, 10 H), 4.65-4.48 (m, 4 H), 3.81 (s, 3 H, OCH3), 3.79 (s, 3 H, OCH3), 3. 69 (s, 3 H, OCH3), 3. (s, 3 H, OCH3), 3.12 (m, 4 H), 3.10-2.67 (m, 8 H), 1.57-1. (m, 4 H). Anal. calcd. for C34H44N2Oa2 : C 60. 70, H 6.59, N 4.16. Found: C 60.64, H 6.61, N 4.15.

EXAMPLE 6 N, N'-Dibenzyl rhizoferrin (7). A solution of (6) (170 mg, 0.253 mmol) in CH30H (7 ml) and 1 N NaOH (7 ml) was stirred at room temperature for 5 hours. HC1 (1 N, 8 ml) was added and the solution was concentrated to about 15 ml. After extraction with EtOAc (3 x 15 ml), the organic layer was dried (Na2SO4) and concentrated to give 120 mg (77%) of (7) as a colorless glass: [a] +12.27 (c 1.00) ; 1H NMR (CD30D) 6 7.42-7.20 (m, 10 H, 2 Ph), 4.67-4.47 (m, 4 H, CH2Ph), 3.35-3.23 (m, 4 H, 2 NCH2), 3.19-2.69 (m, 8 H, 4 CH2CO), 1.58-1.41 (m, 4 H, 2 Ci2). Anal. calcd. for C3oH36N2012HZO : C 56.78, H 6.04, N 4.41. Found: C 56.88, H 6.08, N 4.34.

EXAMPLE 7 Rhizoferrin. A solution of (7) (110 mg, 0.178 mmol) in distilled THF (1.5 ml) was added to Li (33 mg, 4.8 mmol) in NH3 (100 ml) and the mixture was maintained at-78°C for 3 hours. Aqueous H30H (50%, 10 ml) was added until the blue

color disappeared. Ammonia was evaporated and the residue was taken up in water (50 ml) through a cation exchange resin column (Bio Rad, AG 50W-X8). The eluant containing product (pH = 3) was extracted with EtOAc (50 ml) which was concen- trated to dryness. The residue was dissolved in distilled EtOH (2 ml), filtered and concentrated to yield 50 mg (64%) of rhizoferrin as a colorless glass: HRMS (FAB, m-nitrobenzyl alcohol matrix) calcd. for C16H2sN2Ol2 437. 1407 (M + H), found 437. 1407 (base). Anal. calcd. for C16H 24N2012 *H20 : 42. 29, H 5.77, N 6.17. Found: C 42.49, H 5.80, N 5.84.

A solution of crude product (10 mg) was purified by reversed-phase HPLC [Drechsel et al, 1992, supra] (C-18 preparative column, 21.4 mm x 25 cm, obtained from Rainin).

The initial mobile phase concentration of 3% CH3CN in 0.1% TFA was held for 15 minutes, followed by gradient elution of 3-11% CH3CN in 0. TFA over 35 minutes, then held at 11% CH3CN in 0.1% TFA for 20 minutes. Flow rate was maintained at 4 ml per minute. Retention time was 56 minutes. Lyophilization gave 4.32 mg (9.90 pmol) of purified rhizoferrin as a colorless glass: [a]-16.7 (26°C) (c 0.1613) ; 1H NMR (D2O) 6 3.21-3.15 (m, 4 H), 3.02 (d, 2 H, J = 16.0 Hz), 2.79 (d, 2 H, J = 16.0 Hz), 2.76 (d, 2 H, J = 14.6 Hz), 2.65 (d, 2 H, J = 14.6 Hz), 1.53-1.47 (m, 4 H).

A stock solution was prepared by dissolving the purified product in 50.00 ml distilled water; a 10.00 ml ali- quot was diluted to 20.00 ml and adjusted to pH = 3.02 with 1.90 ml of 0.010 N HC1 (final rhizoferrin concentration = 9.04

x 10-5 M). CD and UV spectra were taken immediately after pH adjustment. All spectra were baseline corrected with a dis- tilled water blank which was acidified as above.

CD Results The CD spectra of rhizoferrin exhibited a negative Cotton effect from 200 to 220 nm, with a single minimum at 205 nm, Ae =-2.7 compared to a recorded single minimum [Drechsel et al, 1992, supra at 204 nm, Ae =-4.3.

UV Results nm e £10 196 12200 (13900) 200 10800 (13150) 210 5230 (5600) 215 2770 (3000) 220 1200 (1400)