Title:
METHOD FOR SYNTHESIZING ENZYME FOR β-GLYCOSIDE OF LACTO-N-BIOSE I OR GALACTO-N-BIOSE USING MODIFIED PHOSPHORYLASE
Document Type and Number:
WIPO Patent Application WO/2020/040257
Kind Code:
A1
Abstract:
This application provides: a modified GLNBP protein in which a mutation is introduced to a certain region of a non-mutant GLNBP; and a method of forming a β-1,3 bond between the 1-position of a galactose-1-phosphate using the modified GLNBP, and the 3-position of an N-acetylglucosamine residue or an N-acetylgalactosamine residue in a saccharide having the N-acetylglucosamine residue or the N-acetylgalactosamine residue which is β-bound to a non-reducing end thereof, and also having one or multiple saccharide residues.
Inventors:
KATAYAMA TAKANE (JP)
KATOH TOSHIHIKO (JP)
KITAOKA MOTOMITSU (JP)
KATOH TOSHIHIKO (JP)
KITAOKA MOTOMITSU (JP)
Application Number:
PCT/JP2019/032866
Publication Date:
February 27, 2020
Filing Date:
August 22, 2019
Export Citation:
Assignee:
UNIV KYOTO (JP)
NAT AGRICULTURE & FOOD RES ORG (JP)
NAT AGRICULTURE & FOOD RES ORG (JP)
International Classes:
C12N9/10; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/54; C12N15/63; C12P19/28
Foreign References:
| JPH11253191A | 1999-09-21 | |||
| JP2014168404A | 2014-09-18 | |||
| JP2014187886A | 2014-10-06 |
Other References:
DERENSY-DRON D. ET AL.: "beta -1, 3-Galactosyl-N-acetylhexosamine phosphorylase from Bifidobacterium bifidum DSM 20082 : characterization, partial purification and relation to mucin degradation", BIOTECHNOL. APPL. BIOCHEM., vol. 29, 1999, pages 3 - 10
KITAOKA MOTOMISHU: "Advanced utilization of carbohydrate-processing enzymes", BULLETIN OF APPLIED GLYCOSCIENCE, vol. 8, no. 1, February 2018 (2018-02-01), pages 20 - 32
SAIKI RYOHEI: "Micro-flow synthesis of Lacto-N tetraose", ABSTRACTS OF THE 34TH ANNUAL MEETING OF THE JAPANESE SOCIETY OF CARBOHYDRATE RESEARCH, 1 July 2015 (2015-07-01), pages 250
HUANG WEICHUN ET AL: "Synthesis of Lacto-N-tetraose by the chemical enzyme method", LECTURE PREPRINTS OF JOINT MEETING OF THE TOHOKU AREA CHEMISTRY SOCIETIES 2011, vol. 23, 17 September 2011 (2011-09-17), pages 116
NORIOKA TSUBASA ET AL: "Development of enzymatic synthesis of lacto-N-tetraose using mutation galacto-N-biose/lacto-N-biose I phosphorylase", LECTURE ABSTRACTS OF THE 2019 CONFERENCE OF JSBBA, 5 March 2019 (2019-03-05)
KITAOKA MOTOMISHU: "Advanced utilization of carbohydrate-processing enzymes", BULLETIN OF APPLIED GLYCOSCIENCE, vol. 8, no. 1, February 2018 (2018-02-01), pages 20 - 32
SAIKI RYOHEI: "Micro-flow synthesis of Lacto-N tetraose", ABSTRACTS OF THE 34TH ANNUAL MEETING OF THE JAPANESE SOCIETY OF CARBOHYDRATE RESEARCH, 1 July 2015 (2015-07-01), pages 250
HUANG WEICHUN ET AL: "Synthesis of Lacto-N-tetraose by the chemical enzyme method", LECTURE PREPRINTS OF JOINT MEETING OF THE TOHOKU AREA CHEMISTRY SOCIETIES 2011, vol. 23, 17 September 2011 (2011-09-17), pages 116
NORIOKA TSUBASA ET AL: "Development of enzymatic synthesis of lacto-N-tetraose using mutation galacto-N-biose/lacto-N-biose I phosphorylase", LECTURE ABSTRACTS OF THE 2019 CONFERENCE OF JSBBA, 5 March 2019 (2019-03-05)
Attorney, Agent or Firm:
YAMAO, Norihito et al. (JP)
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