Title:
METHOD FOR SYNTHESIZING L-ASPARTIC ACID BY MEANS OF WHOLE CELL CATALYSIS OF MALEIC ACID BY COUPLING TWO ENZYMES
Document Type and Number:
WIPO Patent Application WO/2019/119614
Kind Code:
A1
Abstract:
Disclosed in the present invention is a method for synthesizing L-aspartic acid by means of the whole cell catalysis of maleic acid by coupling two enzymes, which relates to the field of bio-engineering. In the present invention, a recombinant strain that coupledly expresses a cis-trans isomerase of maleic acid and L-aspartate lyase is constructed, and the recombinant strain is further modified and optimized to obtain a recombinant bacterial strain which may perform whole cell high-conversion rate catalysis of maleic acid to generate L-aspartic acid. The recombinant strain may produce L-aspartic acid by using maleic acid which is relatively inexpensive; after reacting for 40-120 min, the maleic acid has completely reacted, and almost none of the intermediate product of fumaric acid has accumulated, wherein the conversion rate may reach 98% or higher.
More Like This:
Inventors:
ZHOU ZHEMIN (CN)
YU LONG (CN)
ZHOU LI (CN)
CUI WENJING (CN)
LIU ZHONGMEI (CN)
GUO JUNLING (CN)
YU LONG (CN)
ZHOU LI (CN)
CUI WENJING (CN)
LIU ZHONGMEI (CN)
GUO JUNLING (CN)
Application Number:
PCT/CN2018/074337
Publication Date:
June 27, 2019
Filing Date:
January 26, 2018
Export Citation:
Assignee:
UNIV JIANGNAN (CN)
International Classes:
C12P13/20; C12N1/21; C12R1/19
Foreign References:
| CN106636052A | 2017-05-10 | |||
| JP2000139466A | 2000-05-23 | |||
| CN106222122A | 2016-12-14 | |||
| CN108103120A | 2018-06-01 |
Attorney, Agent or Firm:
CENTRAL SOUTH WELL INTELLECTUAL PROPERTY OFFICE (CN)
Download PDF:
Previous Patent: ELECTROLUMINESCENT MATERIAL INK AND ELECTROLUMINESCENT DEVICE THEREOF
Next Patent: METHOD FOR QUANTIFYING TASTE OF WINE
Next Patent: METHOD FOR QUANTIFYING TASTE OF WINE