Title:
METHOD AND SYSTEM FOR ANALYZING N-LINKED SUGAR CHAINS OF GLYCOPROTEIN
Document Type and Number:
WIPO Patent Application WO/2018/034346
Kind Code:
A1
Abstract:
Disclosed is a novel means for accurately performing qualitative and quantitative analysis of each of the linkage sites of N-linked sugar chains. In a method for analyzing N-linked sugar chains of glycoprotein according to the present invention, a portion of a glycopeptide-containing sample to be analyzed is treated with endo-β-N-acetylglucosaminidase, sugar chains are cleaved to leave only a single GlcNAc of a chitobiose core on an Asn linked to N-linked sugar chains, the sugar chain-cleaved sample is pre-analyzed using liquid chromatography/mass spectrometry, and the liquid chromatography retention time of the target glycopeptide to be analyzed and m/z of precursor ions are predicted on the basis of the results of the pre-analysis to perform a main analysis. Thereby, the linkage sites and structures of N-linked sugar chains linked to the glycoprotein can be analyzed. By using the sugar chain-cleaved sample as an internal standard in the main analysis, each of the sugar chain linkage sites of sugar chains can also be quantitatively analyzed.
Inventors:
OHTA YUKI (JP)
KAWASAKI NANA (JP)
TAKAKURA DAISUKE (JP)
KAWASAKI NANA (JP)
TAKAKURA DAISUKE (JP)
Application Number:
PCT/JP2017/029658
Publication Date:
February 22, 2018
Filing Date:
August 18, 2017
Export Citation:
Assignee:
PUBLIC UNIV CORP YOKOHAMA CITY UNIV (JP)
International Classes:
C12Q1/34; C07K7/06; G01N27/62
Domestic Patent References:
| WO2015152135A1 | 2015-10-08 |
Foreign References:
| JP2008309501A | 2008-12-25 | |||
| JP2010256101A | 2010-11-11 | |||
| JP2015145840A | 2015-08-13 | |||
| JP2008051552A | 2008-03-06 | |||
| JP2011505849A | 2011-03-03 | |||
| JP2015142555A | 2015-08-06 | |||
| JP2009145169A | 2009-07-02 |
Other References:
MINAMI MAEHARA ET AL.: "2P-47: Shinkinai Hyojun Busshitsu o Mochiita Kotai Iyakuhin To no Tosa Teiryoho no Kaihatsu [Quantitation method for glycoforms of monoclonal antibodies using a novel internal standard]", DAI 65 KAI ANNUAL CONFERENCE ON MASS SPECTROMETRY (2017); WED, MAY. 17 2017～FRI, MAY. 19 2017 AT 17:00 (JST) , 1 May 2017 (2017-05-01), Japan, pages 160, XP009513508
KAJI, H. ET AL.: "Lectin affinity capture, isotope-coded tagging and mass spectrometry to identify N-linked glycoproteins", NAT BIOTECHNOL, vol. 21, 2003, pages 667 - 672, XP002347925, DOI: doi:10.1038/nbt829
HARAZONO AKAWASAKI NITOH SHASHII NMATSUISHI-NAKAJIMA YKAWANISHI TYAMAGUCHI T: "Simultaneous glycosylation analysis of human serum glycoproteins by high-performance liquid chromatography/tandem mass spectrometry", J. CHROMATOGR. B., vol. 868, 2008, pages 20 - 30, XP022714792, DOI: doi:10.1016/j.jchromb.2008.05.006
See also references of EP 3517623A4
KAJI, H. ET AL.: "Lectin affinity capture, isotope-coded tagging and mass spectrometry to identify N-linked glycoproteins", NAT BIOTECHNOL, vol. 21, 2003, pages 667 - 672, XP002347925, DOI: doi:10.1038/nbt829
HARAZONO AKAWASAKI NITOH SHASHII NMATSUISHI-NAKAJIMA YKAWANISHI TYAMAGUCHI T: "Simultaneous glycosylation analysis of human serum glycoproteins by high-performance liquid chromatography/tandem mass spectrometry", J. CHROMATOGR. B., vol. 868, 2008, pages 20 - 30, XP022714792, DOI: doi:10.1016/j.jchromb.2008.05.006
See also references of EP 3517623A4
Attorney, Agent or Firm:
TANIGAWA AND PARTNERS, PATENT FIRM (JP)
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