Title:
METHOD AND SYSTEM FOR PEAK PARKING IN LIQUID CHROMATOGRAPHY-MASS SPECTROMETER (LC-MS) ANALYSIS
Document Type and Number:
WIPO Patent Application WO2002029399
Kind Code:
A3
Abstract:
A reconfigurable multi-mode post-column analysis system improves resolution of liquid chromatography detection data by providing a parking or MS/MS detection mode in which flow of column eluent is substantially reduced to provide greater analysis time within a single eluting detection peak. A micro switching valve unit is reconfigurable between normal (MS) mode that passes column eluent directly to an MS unit, and a peak parking (or MS/MS) mode, in which normal mode column flow is interrupted, and the gradient halted, while more slowly flowing the detection peak of interested through the MS unit. A micro syringe pump contributes to the substantially lower MS/MS flow rate. A MS control unit controls micro switching valve valves to reconfigure between MS and MS/MS modes. After a detection peak, MS mode is reestablished, and the system primary pump returns to normal mode flow rate and gradient conditions. Mode changes can be carried out for each chromatogram detection peak.
More Like This:
| JP2023118717 | ANALYSIS METHOD AND ANALYZER |
| JP5235279 | Ion collector |
Inventors:
CHERVET JEAN-PIERRE
SWART REMCO
VISSERS JOHANNES PETRUS CORNEL
SWART REMCO
VISSERS JOHANNES PETRUS CORNEL
Application Number:
PCT/US2001/031072
Publication Date:
January 30, 2003
Filing Date:
October 03, 2001
Export Citation:
Assignee:
DIONEX CORP (US)
International Classes:
G01N27/62; G01N30/02; G01N30/26; G01N30/32; G01N30/34; G01N30/72; G01N30/78; G01N30/80; G01N30/84; G01N30/88; G01N30/86; (IPC1-7): G01N30/72; G01N30/32; G01N30/84
Domestic Patent References:
| WO2000011208A1 | 2000-03-02 |
Other References:
ZHOU J ET AL: "QUASI-LINEAR GRADIENTS FOR CAPILLARY LIQUID CHROMATOGRAPHY WITH MASS AND TANDEM MASS SPECTROMETRY", RAPID COMMUNICATIONS IN MASS SPECTROMETRY, HEYDEN, LONDON, GB, vol. 14, no. 6, 2000, pages 432 - 438, XP001041291, ISSN: 0951-4198
DAVIS M T ET AL: "Rapid Protein Identification Using a Microscale Electrospray LC/MS System on an Ion Trap Mass Spectrometer", JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, ELSEVIER SCIENCE INC, US, vol. 9, no. 3, 1 March 1998 (1998-03-01), pages 194 - 201, XP004112014, ISSN: 1044-0305
DAVIS M T ET AL: "Variable Flow Liquid Chromatography-Tandem Mass Spectrometry and the Comprehensive Analysis of Complex Protein Digest Mixtures", JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, ELSEVIER SCIENCE INC, US, vol. 8, no. 10, 1 October 1997 (1997-10-01), pages 1059 - 1069, XP004094061, ISSN: 1044-0305
DAVIS M T ET AL: "Rapid Protein Identification Using a Microscale Electrospray LC/MS System on an Ion Trap Mass Spectrometer", JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, ELSEVIER SCIENCE INC, US, vol. 9, no. 3, 1 March 1998 (1998-03-01), pages 194 - 201, XP004112014, ISSN: 1044-0305
DAVIS M T ET AL: "Variable Flow Liquid Chromatography-Tandem Mass Spectrometry and the Comprehensive Analysis of Complex Protein Digest Mixtures", JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, ELSEVIER SCIENCE INC, US, vol. 8, no. 10, 1 October 1997 (1997-10-01), pages 1059 - 1069, XP004094061, ISSN: 1044-0305
Download PDF: