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Title:
METHOD OF TARGETING MISMATCHED DNA USING D8 SQUARE PLANAR METAL COMPLEXES
Document Type and Number:
WIPO Patent Application WO/2016/206583
Kind Code:
A1
Abstract:
Disclosed are methods of using d8 square planar metal complexes containing tridentate π-conjugated ligands and ancillary ligand of N-heterocyclic carbene or di-phosphine ligand to target mismatched DNA. Targeting of mismatched DNA can be revealed by monitoring the differences in emission enhancement of metal complexes toward mismatched DNA and matched DNA; or the gradually heat release from isothermal titration calorimetry (ITC) when complexes bind toward mismatch DNA; or the significant increase in melting temperature of the mismatched DNA after adding complexes.

Inventors:
CHE, Chi Ming (Flat 5, 5th Floor Block A, Parkway Court, 4 Park Road, Hong Kong, Kong, CN)
FUNG, Sin Ki (Flat H, 19th Floor Block 8, Flora Plaza,Fanling, New Territories, Hong Kong, Kong, CN)
ZOU, Taotao (Number 25B, 13th Floor Kwan Yick Building, Phase 1, 430-440 Des Voeux Road West,Sheung Wan, Hong Kong, Kong, CN)
Application Number:
CN2016/086669
Publication Date:
December 29, 2016
Filing Date:
June 22, 2016
Export Citation:
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Assignee:
THE UNIVERSITY OF HONG KONG (Pokfulam Road, Hong Kong, Kong, CN)
International Classes:
C07F1/12; C07F17/02; C12Q1/68; G01N21/63
Domestic Patent References:
WO2011050574A12011-05-05
Other References:
FUNG, S. K. ET AL.: "Luminescent platinum(II) complexes with functionalized N-heterocyclic carbene or diphosphine selectively probe mismatched and abasic DNA.", NAT. COMMUN., vol. 7, no. 10655, 17 February 2016 (2016-02-17), pages 1 - 9, XP055341513
SONG, H. ET AL.: "Crystal structure of DELTA-[Ru(bpy)2dppz]2+ bound to mismatched DNA reveals side-by-side metalloinsertion and intercalation.", NAT. CHEM., vol. 4, 10 June 2012 (2012-06-10), pages 615 - 620, XP055341517
SUN, R. W. Y. ET AL.: "Luminescent cyclometalated platinum(II) complexes containing N- heterocyclic carbene ligands with potent in vitro and in vivo anti-cance properties accumulate in cytoplasmic structures of cancer cells.", CHEM. SCI., vol. 2, 21 January 2011 (2011-01-21), pages 728 - 736, XP055251177, DOI: doi:10.1039/c0sc00593b
YAN, J. J. ET AL.: "Cyclometalated gold(III) complexes with N-heterocyclic carbene ligands as topoisomerase I poisons.", CHEM. COMMUN., vol. 46, 19 April 2010 (2010-04-19), pages 3893 - 3895, XP002715662
SUN, R. W. Y. ET AL.: "Some uses of transition metal complexes as anti-cancer and anti-HIV agents.", DALTON TRANS., 18 September 2007 (2007-09-18), pages 4884 - 4892, XP055341525
CISNETTI, F. ET AL.: "Metal/N-Heterocyclic Carbene Complexes: Opportunities for the Development of Anticancer Metallodrugs.", ANGEW. CHEM. INT. ED., vol. 52, 2 October 2013 (2013-10-02), pages 11976 - 11978, XP055250261
Attorney, Agent or Firm:
CHINA PATENT AGENT (H.K.) LTD. (22/F, Great Eagle Center,23 Harbour Road,,Wanchai, Hong Kong, Kong, CN)
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