Title:
METHOD FOR TESTING DRUG RESPONSIVENESS OF CARDIOMYOCYTES
Document Type and Number:
WIPO Patent Application WO/2019/131806
Kind Code:
A1
Abstract:
Provided is a method for testing the drug responsiveness of cardiomyocytes, wherein the method comprises: testing the cardiomyocytes for responsiveness to an added drug under conditions where, in a culture liquid, the distance from a liquid surface of the culture liquid to a culture vessel bottom surface with which the cardiomyocytes are in contact is 5.0 mm or less; or testing the cardiomyocytes for responsiveness to an added drug immediately after placement of the cardiomyocytes under conditions where, in a culture liquid, the distance from a liquid surface of the culture liquid to a culture vessel bottom surface with which the cardiomyocytes are in contact is 5.0 mm or less.
Inventors:
SUETA SHINICHI (JP)
MINAMI ITSUNARI (JP)
KASAHARA TOMOKO (JP)
ISHIDA KENTARO (JP)
MAKITA NAOHIRO (JP)
MINAMI ITSUNARI (JP)
KASAHARA TOMOKO (JP)
ISHIDA KENTARO (JP)
MAKITA NAOHIRO (JP)
Application Number:
PCT/JP2018/047956
Publication Date:
July 04, 2019
Filing Date:
December 26, 2018
Export Citation:
Assignee:
MYORIDGE CO LTD (JP)
International Classes:
C12Q1/02; G01N33/15; G01N33/50; C12N5/071; C12N5/10
Domestic Patent References:
| WO2011122200A1 | 2011-10-06 | |||
| WO2015182765A1 | 2015-12-03 | |||
| WO2012117688A1 | 2012-09-07 |
Foreign References:
| JP2013543726A | 2013-12-09 |
Other References:
ANDO, H. ET AL.: "A new paradigm for drug-induced torsadogenic risk assessment using human iPS cell -derived cardiomyocytes", J. PHARMACOL. TOXICOL. METHODS., vol. 84, December 2016 (2016-12-01), pages 111 - 127, XP029953101, ISSN: 1056-8719
AUZUKI, TAKAHIKO ET AL.: "Effective Oxygen Supply stimulates the Beating of Cultured Cardiac Myocytes in a Serum-Free Medium", TISSUE CULTURE RESEARCH COMMUNICATIONS: THE JOURNAL OF EXPERIMENTAL & APPLIED CELL CULTURE RESEARCH, vol. 14, no. 1, 1995, pages 61 (19), XP009521631
MAEYAMA, ERINA ET AL.: "O-26-5: Construction of a new culture technique to achieve efficient oxygen supply", REGENERATIVE MEDICINE, vol. 13, no. Suppl. 1, 2014, pages 222, XP009521630, ISSN: 1347-7919
CARRIER, RL. ET AL.: "Effects of oxygen on engineered cardiac muscle", BIOTECHNOL. BIOENG., vol. 78, no. 6, 2002, pages 617 - 625, XP055623440, ISSN: 0006-3592
J PHARMACOL TOXICOL METHODS., vol. 84, March 2017 (2017-03-01), pages 111 - 127
See also references of EP 3733863A4
AUZUKI, TAKAHIKO ET AL.: "Effective Oxygen Supply stimulates the Beating of Cultured Cardiac Myocytes in a Serum-Free Medium", TISSUE CULTURE RESEARCH COMMUNICATIONS: THE JOURNAL OF EXPERIMENTAL & APPLIED CELL CULTURE RESEARCH, vol. 14, no. 1, 1995, pages 61 (19), XP009521631
MAEYAMA, ERINA ET AL.: "O-26-5: Construction of a new culture technique to achieve efficient oxygen supply", REGENERATIVE MEDICINE, vol. 13, no. Suppl. 1, 2014, pages 222, XP009521630, ISSN: 1347-7919
CARRIER, RL. ET AL.: "Effects of oxygen on engineered cardiac muscle", BIOTECHNOL. BIOENG., vol. 78, no. 6, 2002, pages 617 - 625, XP055623440, ISSN: 0006-3592
J PHARMACOL TOXICOL METHODS., vol. 84, March 2017 (2017-03-01), pages 111 - 127
See also references of EP 3733863A4
Attorney, Agent or Firm:
KURATA, Masatoshi et al. (JP)
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