Title:
METHOD FOR THREE-DIMENSIONAL CULTURE OF CELLS USING FIBER-ON-FIBER AND SUBSTRATE THEREFOR
Document Type and Number:
WIPO Patent Application WO/2014/196549
Kind Code:
A1
Abstract:
The present invention provides, for example, the following: a substrate for cell culture, which is a support on which are contained nanofibers formed from a biopolymer selected from the group consisting of gelatin, collagen, and cellulose; a cell cryogen containing the substrate; a method for cell culture comprising inoculation of cells onto the substrate and static culture of the cells; the same method comprising dissociation of the cells from the substrate using an enzyme-free dissociation solution, inoculation of the cells onto another of the culture substrate, and further static culture of the cells; in particular, a method comprising separation of subcultured cells into single cells; and a method for cryopreservation of pluripotent stem cells using the cell cryogen.
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Inventors:
KAMEI KENICHIRO (JP)
NAKATSUJI NORIO (JP)
LIU LI (JP)
NAKATSUJI NORIO (JP)
LIU LI (JP)
Application Number:
PCT/JP2014/064789
Publication Date:
December 11, 2014
Filing Date:
June 03, 2014
Export Citation:
Assignee:
UNIV KYOTO (JP)
International Classes:
C12M3/00; C12N1/04; C12N5/0735; C12N5/10
Foreign References:
| JP2008514341A | 2008-05-08 |
Other References:
TUZLAKOGLU, K ET AL: "DESIGN OF NANO- AND MICROFIBER COMBINED SCAFFOLDS BY ELECTROSPINNING OF COLLAGEN ONTO STARCH BASED FIBER MESHES: A MAN MADE EQUIVALENT OF NATURAL EXTRACELLULAR MATRIX", TISSUE ENGINEERING PART A, vol. 17, no. 3-4, 2011, pages 463 - 473
Attorney, Agent or Firm:
TAKASHIMA, HAJIME (JP)
Takashima 1 (JP)
Takashima 1 (JP)
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