Title:
METHOD TO DETECT PROSTATE CANCER IN A SAMPLE
Document Type and Number:
WIPO Patent Application WO2004070056
Kind Code:
A3
Abstract:
The present invention relates to prostate cancer. More specifically, the present invention relates to a method to detect prostate cancer in a patient sample by detecting the RNA encoded by the gene PCA3. More particularly the present invention relates to a method for determining a predisposition, or presence of prostate cancer in a patient comprising: (a) contacting a biological sample of a patient with at least one oligonucleotide that hybridizes to a PCA3 polynucleotide; (b) detecting in the biological sample an amount of PCA3 and second prostate specific polynucleotides; and (c) comparing the amount of PCA3 polynucleotide that hybridizes to the oligonucleotide to a predetermined cut off value, and therefrom determining the presence or absence of prostate cancer in the biological sample. The present invention further relates to diagnostic kits for the detection of prostate cancer or the risk of developing same in a patient comprising: (a) at least one container means having disposed therein at least one oligonucleotide probe or primer that hybridizes to one a PCA3 nucleic acid or complement thereof; (b) at least one oligonucleotide probe or primer that hybridizes with a second prostate pecific nucleic acid or complement thereof; and (c) reagents enabling a detection of PCA3 and of the second prostate specific nucleic acid when PCA3 or second prostate-specific nucleic acid sequence is present.
Inventors:
FRADET YVES (CA)
CHYPRE CAMILLE (CA)
PICHE LYSON (CA)
GARON GENEVIEVE (CA)
CHYPRE CAMILLE (CA)
PICHE LYSON (CA)
GARON GENEVIEVE (CA)
Application Number:
PCT/CA2004/000170
Publication Date:
September 30, 2004
Filing Date:
February 09, 2004
Export Citation:
Assignee:
DIAGNOCURE INC (CA)
FRADET YVES (CA)
CHYPRE CAMILLE (CA)
PICHE LYSON (CA)
GARON GENEVIEVE (CA)
FRADET YVES (CA)
CHYPRE CAMILLE (CA)
PICHE LYSON (CA)
GARON GENEVIEVE (CA)
International Classes:
C12Q1/68; (IPC1-7): C12Q1/68
Domestic Patent References:
| WO1998045420A1 | 1998-10-15 | |||
| WO2001023550A2 | 2001-04-05 | |||
| WO2000058470A1 | 2000-10-05 | |||
| WO2000050899A1 | 2000-08-31 |
Other References:
KOK DE J B ET AL: "DD3PCA3, A VERY SENSITIVE AND SPECIFIC MARKER TO DETECT PROSTATE TUMORS", CANCER RESEARCH, AMERICAN ASSOCIATION FOR CANCER RESEARCH, BALTIMORE, MD, US, vol. 62, no. 9, 1 May 2002 (2002-05-01), pages 2695 - 2698, XP001189400, ISSN: 0008-5472
BUSSEMAKERS M J ET AL: "DD3: a new prostate-specific gene, highly overexpressed in prostate cancer.", CANCER RESEARCH. UNITED STATES 1 DEC 1999, vol. 59, no. 23, 1 December 1999 (1999-12-01), pages 5975 - 5979, XP002284051, ISSN: 0008-5472
TAMIMI Y ET AL: "DiagnoGene PCA3 reliable NASBA based reagents for detecting PCA3 mRNA, a recently described prostate marker", PROCEEDINGS OF THE AMERICAN ASSOCIATION FOR CANCER RESEARCH ANNUAL, vol. 39, March 1998 (1998-03-01), 89th Annual Meeting of the American Association for Cancer Research;New Orleans, Louisiana, USA; March 28-April 1, 1998, March, 1998, pages 234, XP001181827, ISSN: 0197-016X
VERHAEGH G W ET AL: "Isolation and characterization of the promoter of the human prostate cancer-specific DD3 gene.", THE JOURNAL OF BIOLOGICAL CHEMISTRY. UNITED STATES 1 DEC 2000, vol. 275, no. 48, 1 December 2000 (2000-12-01), pages 37496 - 37503, XP002284052, ISSN: 0021-9258
HESSELS DAPHNE ET AL: "DD3PCA3-based molecular urine analysis for the diagnosis of prostate cancer.", EUROPEAN UROLOGY, vol. 44, no. 1, July 2003 (2003-07-01), pages 8 - 16, XP009032062, ISSN: 0302-2838 (ISSN print)
BUSSEMAKERS M J ET AL: "DD3: a new prostate-specific gene, highly overexpressed in prostate cancer.", CANCER RESEARCH. UNITED STATES 1 DEC 1999, vol. 59, no. 23, 1 December 1999 (1999-12-01), pages 5975 - 5979, XP002284051, ISSN: 0008-5472
TAMIMI Y ET AL: "DiagnoGene PCA3 reliable NASBA based reagents for detecting PCA3 mRNA, a recently described prostate marker", PROCEEDINGS OF THE AMERICAN ASSOCIATION FOR CANCER RESEARCH ANNUAL, vol. 39, March 1998 (1998-03-01), 89th Annual Meeting of the American Association for Cancer Research;New Orleans, Louisiana, USA; March 28-April 1, 1998, March, 1998, pages 234, XP001181827, ISSN: 0197-016X
VERHAEGH G W ET AL: "Isolation and characterization of the promoter of the human prostate cancer-specific DD3 gene.", THE JOURNAL OF BIOLOGICAL CHEMISTRY. UNITED STATES 1 DEC 2000, vol. 275, no. 48, 1 December 2000 (2000-12-01), pages 37496 - 37503, XP002284052, ISSN: 0021-9258
HESSELS DAPHNE ET AL: "DD3PCA3-based molecular urine analysis for the diagnosis of prostate cancer.", EUROPEAN UROLOGY, vol. 44, no. 1, July 2003 (2003-07-01), pages 8 - 16, XP009032062, ISSN: 0302-2838 (ISSN print)
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