Title:
A METHOD TO PRODUCE ACETYLDIACYLGLYCEROLS (AC-TAGS) BY EXPRESSION OFAN ACETYLTRANSFERASE GENE ISOLATED FROM EUONYMUS ALATUS (BURNING BUSH)
Document Type and Number:
WIPO Patent Application WO/2011/082253
Kind Code:
A3
Abstract:
The present invention relates to novel diacylglycerol acyltransferase genes and proteins, and methods of their use. In particular, the invention describes genes encoding proteins having diacylglycerol acetyltransferase activity, specifically for transferring an acetyl group to a diacylglycerol substrate to form acetyl- Triacylglycerols (ac-TAGs), for example, a 3-acetyl-1,2-diacyl-sn-glycerol. The present invention encompasses both native and recombinant wild-type forms of the transferase, as well as mutants and variant forms. The present invention also relates to methods of using novel diacylglycerol acyltransferase genes and proteins, including their expression in transgenic organisms at commercially viable levels, for increasing production of 3 -acetyl- 1,2-diacyl-sn-glycerols in plant oils and altering the composition of oils produced by microorganisms, such as yeast, by increasing ac- TAG production. Additionally, oils produced by methods of the present inventions comprising genes and proteins are contemplated for use as biodiesel fuel, in polymer production and as naturally produced food oils with reduced calories.
Inventors:
DURRETT TIMOTHY (US)
OHLROGGE JOHN (US)
POLLARD MICHAEL (US)
OHLROGGE JOHN (US)
POLLARD MICHAEL (US)
Application Number:
PCT/US2010/062407
Publication Date:
September 29, 2011
Filing Date:
December 29, 2010
Export Citation:
Assignee:
UNIV MICHIGAN STATE (US)
DURRETT TIMOTHY (US)
OHLROGGE JOHN (US)
POLLARD MICHAEL (US)
DURRETT TIMOTHY (US)
OHLROGGE JOHN (US)
POLLARD MICHAEL (US)
International Classes:
C07H21/04; C12N15/82
Foreign References:
| US20070028329A1 | 2007-02-01 | |||
| US20070204370A1 | 2007-08-30 |
Other References:
DATABASE UNIPROT [online] 15 December 2009 (2009-12-15), retrieved from http://www.uniprot.org/uniprotB9SS04.txt?version=5 Database accession no. B9SSQ4_RICCO.
KNOTHE ET AL.: "Kinematic viscosity of biodiesel fuel components and related compounds. Influence of compound structure and comparison to petrodiesel fuel components.", FUEL, vol. 84, no. 9, 2005, pages 1059 - 1065
DATABASE UNIPROT [online] 13 July 2010 (2010-07-13), retrieved from http://www.uniprot.org/uniprot/D6NSS8.txt Database accession no. D6NSS8_EUOAL.
DURRETT ET AL.: "A distinct DGAT with sn-3 acetyltransferase activity that synthesizes unusual, reduced-viscosity oils in Euonymus and transgenic seeds.", PROC NATL ACAD SCI USA, vol. 107, no. 20, 18 May 2010 (2010-05-18), pages 9464 - 9469
KNOTHE ET AL.: "Kinematic viscosity of biodiesel fuel components and related compounds. Influence of compound structure and comparison to petrodiesel fuel components.", FUEL, vol. 84, no. 9, 2005, pages 1059 - 1065
DATABASE UNIPROT [online] 13 July 2010 (2010-07-13), retrieved from http://www.uniprot.org/uniprot/D6NSS8.txt Database accession no. D6NSS8_EUOAL.
DURRETT ET AL.: "A distinct DGAT with sn-3 acetyltransferase activity that synthesizes unusual, reduced-viscosity oils in Euonymus and transgenic seeds.", PROC NATL ACAD SCI USA, vol. 107, no. 20, 18 May 2010 (2010-05-18), pages 9464 - 9469
Attorney, Agent or Firm:
CARROLL, Peter, G. et al. (101 Howard Street Suite 35, San Francisco CA, US)
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