Title:
METHOD TO SUPPRESS DEDIFFERENTIATION OF CELLS THAT READILY DEDIFFERENTIATE, METHOD FOR PREPARING SAID CELLS, AND METHOD FOR PRODUCING SUBSTANCE
Document Type and Number:
WIPO Patent Application WO/2018/021362
Kind Code:
A1
Abstract:
The present invention relates to a method to suppress the dedifferentiation of cells that readily dedifferentiate, said method including: (1) a step in which the cells are applied to a polymer porous film; and (2) a step in which the cells are cultivated and allowed to multiply, wherein the polymer porous film is a polymer porous film with a three-layer structure, having a surface layer A and a surface layer B that have a plurality of holes, and a macrovoid layer that is sandwiched between the surface layer A and the surface layer B, the average hole diameter of the holes present in the surface layer A is smaller than the average hole diameter of the holes present in the surface layer B, the macrovoid layer has dividing walls that are connected to the surface layers A and B, and a plurality of macrovoids that are surrounded by the dividing walls and the surface layers A and B, and the holes in the surface layers A and B are in communication with the macrovoids.
Inventors:
HAGIHARA MASAHIKO (JP)
KAWAGUCHI TETSUO (JP)
BABA KOUSUKE (JP)
KAWAGUCHI TETSUO (JP)
BABA KOUSUKE (JP)
Application Number:
PCT/JP2017/026942
Publication Date:
February 01, 2018
Filing Date:
July 25, 2017
Export Citation:
Assignee:
UBE INDUSTRIES (JP)
International Classes:
C12N5/07; C12P21/02; C12M3/00
Domestic Patent References:
WO2015012415A1 | 2015-01-29 | |||
WO2005095577A1 | 2005-10-13 |
Foreign References:
JP2011078710A | 2011-04-21 | |||
JP2012000262A | 2012-01-05 | |||
JP2015198645A | 2015-11-12 | |||
JP2010539905A | 2010-12-24 |
Attorney, Agent or Firm:
AOKI, Atsushi et al. (JP)
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