Title:
METHOD FOR VISUALIZING EUKARYOTIC CELL, AND MODIFIED REPORTER GENE AND EXPRESSION VECTOR FOR VISUALIZING EUKARYOTIC CELL
Document Type and Number:
WIPO Patent Application WO/2016/117135
Kind Code:
A1
Abstract:
Provided are a method for visualizing an eukaryotic cell, whereby the eukaryotic cell can be specifically identified from a sample in which the eukaryotic cell is mixed with a prokaryote, and a modified reporter gene.
The method according to the present invention for visualizing an eukaryotic cell comprises: a step for preparing a sample in which one or more eukaryotic cells are mixed with one or more prokaryotes; a step for introducing one or more modified reporter genes, which contain a sequence encoding a light-related protein and have been modified so that the quantity of light derived from the light-related protein is reduced in the prokaryotes, into the eukaryotic cells and the prokaryotes; a step for placing the sample under such conditions as enabling the photodetection of the light-related protein; and a step for photodetecting the light-related protein in the sample. The modified reporter gene according to the present invention contains a sequence encoding a light-related protein and an intron spliced in an eukaryotic cell, wherein the intron is inserted into the sequence encoding the light-related protein.
Inventors:
GODA KAZUHITO (JP)
Application Number:
PCT/JP2015/052781
Publication Date:
July 28, 2016
Filing Date:
January 23, 2015
Export Citation:
Assignee:
OLYMPUS CORP (JP)
International Classes:
C12Q1/02; C12N15/09
Other References:
S. LUKE MANKIN ET AL.: "Introduction of a plant intron into the luciferase gene of Photinus pyralis", PLANT MOLECULAR BIOLOGY REPORTER, vol. 15, no. 2, 1997, pages 186 - 196
HIDETO HAYAKAWA ET AL.: "Novel intron-containing luciferase genes for quantitative analysis of mRNA levels in transient gene expression assays", PLANT BIOTECHNOLOGY, vol. 29, no. 5, 2012, pages 505 - 509
ERIKO OGURA ET AL.: "Idenshi Hatsugen Kaiseki ni Okeru Intron Sonyugata Hakko Reporter Idenshi no Riyo Oyobi Kohatsugen Vector Cis Hairetsu no Saitekika ni Tsuite", JAPANESE SOCIETY FOR PLANT CELL AND MOLECULAR BIOLOGY TAIKAI SYMPOSIUM KOEN YOSHISHU, vol. 32, 2014, pages 77
ERIKO OGURA ET AL.: "Idenshi Hatsugen Kaiseki ni Okeru Intron Sonyugata Hakko Reporter Idenshi no Oyo", JAPANESE SOCIETY FOR PLANT CELL AND MOLECULAR BIOLOGY TAIKAI SYMPOSIUM KOEN YOSHISHU, vol. 31, 2013, pages 144
JOSE S. GIL ET AL.: "A method to rapidly and accurately compare relative efficacies of non- invasive imaging reporter genes in a mouse model, and its application to luciferase reporters", MOL IMAGING BIOL., 2013, Retrieved from the Internet
HIDETO HAYAKAWA ET AL.: "Novel intron-containing luciferase genes for quantitative analysis of mRNA levels in transient gene expression assays", PLANT BIOTECHNOLOGY, vol. 29, no. 5, 2012, pages 505 - 509
ERIKO OGURA ET AL.: "Idenshi Hatsugen Kaiseki ni Okeru Intron Sonyugata Hakko Reporter Idenshi no Riyo Oyobi Kohatsugen Vector Cis Hairetsu no Saitekika ni Tsuite", JAPANESE SOCIETY FOR PLANT CELL AND MOLECULAR BIOLOGY TAIKAI SYMPOSIUM KOEN YOSHISHU, vol. 32, 2014, pages 77
ERIKO OGURA ET AL.: "Idenshi Hatsugen Kaiseki ni Okeru Intron Sonyugata Hakko Reporter Idenshi no Oyo", JAPANESE SOCIETY FOR PLANT CELL AND MOLECULAR BIOLOGY TAIKAI SYMPOSIUM KOEN YOSHISHU, vol. 31, 2013, pages 144
JOSE S. GIL ET AL.: "A method to rapidly and accurately compare relative efficacies of non- invasive imaging reporter genes in a mouse model, and its application to luciferase reporters", MOL IMAGING BIOL., 2013, Retrieved from the Internet
Attorney, Agent or Firm:
SUGIMURA, KENJI (JP)
Kenji Sugimura (JP)
Kenji Sugimura (JP)
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