Title:
METHOD FOR VISUALLY MARKING GENOMIC SITE AND APPLICATION THEREOF IN DETECTION OF CELL AND ORGANISM AGING LEVELS
Document Type and Number:
WIPO Patent Application WO/2018/133222
Kind Code:
A1
Abstract:
The present invention discloses a method for visually marking a genomic site and an application thereof in the detection of cell and organism aging levels. The present invention integrates thioredoxin (TRX) at the C-terminus of a transcription activator-like effector (TALE) protein to create a visual genome marking tool, TTALE, for marking telomeres, centromeres, rDNA, and other genomic repetitive sequences and encoding loci in different types of human cells. The present invention also discloses a kit and a method for detecting the number of rDNA copies in the genome of human cells to detect or assist in detecting the aging level of a cell or an organism to be tested.
Inventors:
LIU GUANGHUI (CN)
QU JING (CN)
REN RUOTONG (CN)
DENG LIPING (CN)
QU JING (CN)
REN RUOTONG (CN)
DENG LIPING (CN)
Application Number:
PCT/CN2017/080395
Publication Date:
July 26, 2018
Filing Date:
April 13, 2017
Export Citation:
Assignee:
INST BIOPHYSICS CAS (CN)
International Classes:
C12Q1/68; C07K19/00; C12N15/62; C12N15/65; C12N15/85
Other References:
GANLEY, A.R.D. ET AL.: "Ribosomal DNA and Cellular Senescence: New Evidence Supporting the Connection between rDNA and Aging", FEMS YEAST RESEARCH, vol. 14, no. 1, 31 January 2014 (2014-01-31), pages 49 - 59, XP055506029
MAHFOUZ, M.M. ET AL.: "Targeted Transcriptional Repression Using a Chimeric TALE-SR-DX Repressor Protein", PLANT MOLECULAR BIOLOGY, vol. 78, no. 3, 31 March 2012 (2012-03-31), pages 311 - 321, XP035003111
MAHFOUZ, M.M. ET AL.: "Targeted Transcriptional Repression Using a Chimeric TALE-SR-DX Repressor Protein", PLANT MOLECULAR BIOLOGY, vol. 78, no. 3, 31 March 2012 (2012-03-31), pages 311 - 321, XP035003111
Attorney, Agent or Firm:
JEEKAI&PARTNERS (CN)
Download PDF: