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Title:
METHOD FOR WASHING CELLS
Document Type and Number:
WIPO Patent Application WO/2010/073808
Kind Code:
A1
Abstract:
By efficiently washing cells, cells from which unnecessary components have been removed are collected.  A method for washing cells which comprises: a centrifugation step (S1) comprising introducing a cell suspension into a centrifuge container provided with a washing liquor-jetting port facing the bottom, and rotating the container in such a manner that the bottom is radially outwardly directed, to thereby centrifuge and sediment the cells at the bottom; a supernatant-discharging step (S2) for discharging the supernatant separated by the centrifugation in the preceding centrifugation step (S1); and a washing step (S3) for, after discharging the supernatant in the preceding supernatant-discharging step (S2), jetting a washing liquor from the jetting port while rotating the centrifuge container.

Inventors:
TSUCHIDA, Hiroki (43-2 Hatagaya 2-chome, Shibuya-k, Tokyo 72, 〒1510072, JP)
土田 裕基 (〒72 東京都渋谷区幡ヶ谷2丁目43番2号 オリンパス株式会社内 Tokyo, 〒1510072, JP)
KARASAWA, Chisato (43-2 Hatagaya 2-chome, Shibuya-k, Tokyo 72, 〒1510072, JP)
唐澤 千里 (〒72 東京都渋谷区幡ヶ谷2丁目43番2号 オリンパス株式会社内 Tokyo, 〒1510072, JP)
YOSHIDA, Kazuhiro (43-2 Hatagaya 2-chome, Shibuya-k, Tokyo 72, 〒1510072, JP)
Application Number:
JP2009/068237
Publication Date:
July 01, 2010
Filing Date:
October 23, 2009
Export Citation:
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Assignee:
OLYMPUS CORPORATION (43-2, Hatagaya 2-chome Shibuya-k, Tokyo 72, 〒1510072, JP)
オリンパス株式会社 (〒72 東京都渋谷区幡ヶ谷2丁目43番2号 Tokyo, 〒1510072, JP)
Cytori Therapeutics, Inc. (3020, Callan Road San Dieg, California ., 92121, US)
サイトリ セラピューティクス インコーポレイテッド (92121 カリフォルニア州サンディエゴ カランロード 3020 California, 92121, US)
TSUCHIDA, Hiroki (43-2 Hatagaya 2-chome, Shibuya-k, Tokyo 72, 〒1510072, JP)
土田 裕基 (〒72 東京都渋谷区幡ヶ谷2丁目43番2号 オリンパス株式会社内 Tokyo, 〒1510072, JP)
KARASAWA, Chisato (43-2 Hatagaya 2-chome, Shibuya-k, Tokyo 72, 〒1510072, JP)
International Classes:
C12N5/07; C12N1/02; B04B11/04; B04B15/12
Attorney, Agent or Firm:
UEDA, Kunio et al. (37F The Landmark Tower Yokohama, 2-2-1 Minatomirai, Nishi-ku, Yokohama-sh, Kanagawa 37, 〒2208137, JP)
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