Title:
METHODS OF 6-O-SULFATING POLYSACCHARIDES AND 6-O-SULFATED POLYSACCHARIDE PREPARATIONS
Document Type and Number:
WIPO Patent Application WO2002079258
Kind Code:
A3
Abstract:
Disclosed are methods of 6-O-sulfating glucosaminyl N-acetylglucosamine residues (G1cNAc) in a polysaccharide preparation and methods of converting anticoagulant-inactive heparan sulfate to anticoagulant-active heparan sulfate and substantially pure polysaccharide preparations may by such methods. Also disclosed is a mutant CHO cell which hyper-produces anticoagulant-active heparan sulfate. Methods for elucidating the sequence of activity of enzymes in a biosynthetic pathway are provided.
Inventors:
ROSENBERG ROBERT D (US)
ZHANG LIJUAN (US)
BEELER DAVID L (US)
ZHANG LIJUAN (US)
BEELER DAVID L (US)
Application Number:
PCT/US2002/010172
Publication Date:
November 06, 2003
Filing Date:
March 28, 2002
Export Citation:
Assignee:
MASSACHUSETTS INST TECHNOLOGY (US)
ROSENBERG ROBERT D (US)
ZHANG LIJUAN (US)
BEELER DAVID L (US)
ROSENBERG ROBERT D (US)
ZHANG LIJUAN (US)
BEELER DAVID L (US)
International Classes:
A61K8/73; A61Q5/12; A61Q19/00; C08B37/00; C12N15/09; C12N5/10; C12N9/10; C12P19/26; C12Q1/02; C12Q1/68; (IPC1-7): C12P19/04; C12P19/00; C12P11/00; C12Q1/68
Domestic Patent References:
| WO2002000889A1 | 2002-01-03 |
Foreign References:
| JP2000060566A | 2000-02-29 | |||
| JPH1169983A | 1999-03-16 |
Other References:
HABUCHI H. ET AL.: "Molecular characterization and expression of heparan-sulfate 6-sulfotransferase", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 273, no. 15, 10 April 1998 (1998-04-10), pages 9208 - 9213, XP002966777
HABUCHI H. ET AL.: "The occurence of three isoforms of heparan sulfate 6-O-sulfotransferase having different specificities for hexuronic acid adjacent to the target N-sulfoglucosamine", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 275, no. 4, 28 February 2000 (2000-02-28), pages 2859 - 2868, XP002148154
BAI X. ET AL.: "Turnover of heparan sulfate depends on 2-O-sulfation of uronic acids", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, no. 37, 12 September 1997 (1997-09-12), pages 23172 - 23179, XP002966778
BAI X. ET AL.: "An animal cell mutant defective in heparan sulfate hexuronic acid 2-O-sulfation", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 271, no. 30, 26 July 1996 (1996-07-26), pages 17711 - 17717, XP000601211
HABUCHI H. ET AL.: "The occurence of three isoforms of heparan sulfate 6-O-sulfotransferase having different specificities for hexuronic acid adjacent to the target N-sulfoglucosamine", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 275, no. 4, 28 February 2000 (2000-02-28), pages 2859 - 2868, XP002148154
BAI X. ET AL.: "Turnover of heparan sulfate depends on 2-O-sulfation of uronic acids", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, no. 37, 12 September 1997 (1997-09-12), pages 23172 - 23179, XP002966778
BAI X. ET AL.: "An animal cell mutant defective in heparan sulfate hexuronic acid 2-O-sulfation", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 271, no. 30, 26 July 1996 (1996-07-26), pages 17711 - 17717, XP000601211
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