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Title:
METHODS AND APPARATUS FOR TEMPLATE CAPTURE AND NORMALIZATION FOR SUBMICROLITER REACTION
Document Type and Number:
WIPO Patent Application WO2001009389
Kind Code:
A3
Abstract:
Methods for preparing nanoscale reactions using nucleic acids are presented. Nucleic acids are captured saturably, yet reversibly, on the internal surface of the reaction chamber, typically a capillary. Excess nucleic acid is removed and the reaction is performed directly within the capillary. Alternatively, the saturably bound nucleic acid is eluted, dispensing a metered amount of nucleic acid for subsequent reaction in a separate chamber. Devices for effecting the methods of the invention and a system designed advantageously to utilize the methods for high throughput nucleic acid sequencing reactions are also provided.

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Inventors:
HADD ANDY
JOVANOVICH STEVAN
Application Number:
PCT/US2000/021182
Publication Date:
August 16, 2001
Filing Date:
August 02, 2000
Export Citation:
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Assignee:
MOLECULAR DYNAMICS INC (US)
International Classes:
B01D57/02; C12N15/09; B01J4/00; B01J19/00; B01J19/26; B01L7/00; B01L99/00; B03C5/00; C12M1/00; C12M1/34; C12M1/38; C12Q1/68; C40B40/02; C40B50/06; C40B60/04; G01N1/00; G01N27/447; G01N31/20; G01N33/50; G01N35/00; G01N35/02; G01N35/10; G01N37/00; B01L3/00; C40B40/06; C40B60/14; (IPC1-7): C12Q1/68
Domestic Patent References:
WO1997010331A11997-03-20
WO1996001836A11996-01-25
WO1997040939A11997-11-06
Foreign References:
US5846727A1998-12-08
EP0671626A11995-09-13
DE19746874A11999-04-29
US5785926A1998-07-28
US5720923A1998-02-24
EP0649853A11995-04-26
Other References:
SWERDLOW H ET AL: "FULLY AUTOMATED DNA REACTION AND ANALYSIS IN A FLUIDIC CAPILLARY INSTRUMENT", ANALYTICAL CHEMISTRY,US,AMERICAN CHEMICAL SOCIETY. COLUMBUS, vol. 69, no. 5, 1 March 1997 (1997-03-01), pages 848 - 855, XP000197684, ISSN: 0003-2700
HADD ANDREW G ET AL: "Sub-microliter DNA sequencing for capillary array electrophoresis.", JOURNAL OF CHROMATOGRAPHY A, vol. 894, no. 1-2, October 2000 (2000-10-01), pages 191 - 201, XP000982717, ISSN: 0021-9673
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