Title:
METHODS FOR ASSEMBLING MULTIPLE EXPRESSION CONSTRUCTS
Document Type and Number:
WIPO Patent Application WO/2005/108568
Kind Code:
A1
Abstract:
The present invention relates to methods for assembling constructs with multiple gene expression cassettes by directed recombinational cloning. DNA and vectors having engineered recombination sites are provided for use in a recombinational cloning method that enables efficient and specific assembly of expression cassettes to one multiple expression construct in a single recombinational cloning step.
Inventors:
LOYALL LINDA PATRICIA (DE)
Application Number:
PCT/EP2005/004545
Publication Date:
November 17, 2005
Filing Date:
April 28, 2005
Export Citation:
Assignee:
BASF PLANT SCIENCE GMBH (DE)
LOYALL LINDA PATRICIA (DE)
LOYALL LINDA PATRICIA (DE)
International Classes:
C12N15/09; C12N15/10; C12N15/64; C12N15/66; (IPC1-7): C12N15/09; C12N5/10
Domestic Patent References:
| WO2003103600A2 | 2003-12-18 | |||
| WO2002081711A1 | 2002-10-17 | |||
| WO2002086144A2 | 2002-10-31 |
Foreign References:
| US20030175970A1 | 2003-09-18 | |||
| GB2393441A | 2004-03-31 |
Attorney, Agent or Firm:
Bieberbach, Andreas c/o Basf Aktiengesellschaft (Ludwigshafen, DE)
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