Title:
METHODS OF BARCODING NUCLEIC ACID FOR DETECTION AND SEQUENCING
Document Type and Number:
WIPO Patent Application WO/2020/247685
Kind Code:
A3
Abstract:
The present invention provides methods to barcode nucleic acid for detection and sequencing. It applies a barcode template in a compartment with various targets, including nucleic acid fragments, nuclei and/or cells. After clonal amplification within the compartment, barcode sequence will integrate into its targets before the compartment is broken so that it will effectively barcode nucleic acid fragments originated from a nucleic acid fragment, a nucleus or a cell clonally. The barcode information can be used for tracking the origin of the fragment, nucleus or cell and be used for haplotype phasing and a variety of single cell-based applications including whole genome sequencing, targeted sequencing, RNA sequencing and immune repertoire sequencing.
Inventors:
CHEN ZHOUTAO (US)
PORTER DEVIN (US)
MO GUOYA (US)
WU TSAI-CHIN (US)
PORTER DEVIN (US)
MO GUOYA (US)
WU TSAI-CHIN (US)
Application Number:
PCT/US2020/036198
Publication Date:
January 07, 2021
Filing Date:
June 04, 2020
Export Citation:
Assignee:
UNIVERSAL SEQUENCING TECH CORPORATION (US)
International Classes:
C12N15/10; C12N15/11; C12Q1/6804
Foreign References:
| US20180340169A1 | 2018-11-29 | |||
| US20190078150A1 | 2019-03-14 | |||
| US20130217071A1 | 2013-08-22 |
Attorney, Agent or Firm:
WU, George T. (US)
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