METHODS OF CALIBRATING AN IMAGING SYSTEM USING CALIBRATION BEADS

WIPO Patent Application WO2004066189

B1

When utilized in a flow imaging instrument, calibration beads provide a known data source that can be employed in various self diagnostic, calibration, and quality metric applications for the both the optical system of the flow imaging instrument, as well as the flow cell of the flow imaging instrument. Such data can be used to determine point spread functions associated with an imaging system, to determine a sensitivity of an imaging system, and to determine a focal point of the imaging system. Imagery collected from calibration beads can be used to determine core size and stability and TDI/flow speed synchronization. Calibration beads can be beneficially employed to enable stable system operation, even when very low sample concentration, or very small sample sizes are to be analyzed.

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ORTYN WILLIAM (US)
BASIJI DAVID (US)
FROST KEITH (US)
HALL BRIAN (US)
SEO MICHAEL (US)

PCT/US2004/001116

November 04, 2004

January 16, 2004

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AMNIS CORP (US)
ORTYN WILLIAM (US)
BASIJI DAVID (US)
FROST KEITH (US)
HALL BRIAN (US)
SEO MICHAEL (US)

G01N15/10; G01N15/14; G02B27/00; G06T7/20; (IPC1-7): G01P3/36

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