Title:
METHODS, CELLS AND REAGENTS FOR PRODUCTION OF ISOPRENE, DERIVATIVES AND INTERMEDIATES THEREOF
Document Type and Number:
WIPO Patent Application WO/2017/029553
Kind Code:
A3
Abstract:
This application describes methods, including non-naturally occurring methods, for biosynthesizing 3-hydroxy~3-methylgSutaryl-coA and intermediates thereof, as well as non-naturally occurring hosts for producing 3-hydroxy-3-methyigiutaryl-coA. This application also describes methods, including non-naturally occurring methods, for biosynthesizing isoprene and intermediates thereof, as well as non-naturally occurring hosts for producing isoprene.
Inventors:
CONRADIE ALEX VAN ECK (GB)
Application Number:
PCT/IB2016/001245
Publication Date:
April 06, 2017
Filing Date:
August 16, 2016
Export Citation:
Assignee:
CONRADIE ALEX VAN ECK (GB)
International Classes:
C12P5/02; C12N9/02; C12N9/88; C12N15/52; C12P7/42; C12P19/32
Domestic Patent References:
| WO2009064910A2 | 2009-05-22 | |||
| WO2013119340A1 | 2013-08-15 | |||
| WO2013180584A1 | 2013-12-05 |
Foreign References:
| US20120329119A1 | 2012-12-27 |
Other References:
GREGORY M WHITED ET AL: "Development of a gas-phase bioprocess for isoprene-monomer production using metabolic pathway engineering", INDUSTRIAL BIOTECHNOLOGY, vol. 6, no. 3, 1 June 2010 (2010-06-01), pages 152 - 163, XP055284309
MALEA M. KNEEN ET AL: "Characterization of a thiamin diphosphate-dependent phenylpyruvate decarboxylase from Saccharomyces cerevisiae", FEBS JOURNAL, vol. 278, no. 11, 18 June 2011 (2011-06-18), GB, pages 1842 - 1853, XP055318181, ISSN: 1742-464X, DOI: 10.1111/j.1742-4658.2011.08103.x
JOSÉ SALUD RODRÍGUEZ-ZAVALA ET AL: "Characterization of E. coli tetrameric aldehyde dehydrogenases with atypical properties compared to other aldehyde dehydrogenases", PROTEIN SCIENCE, vol. 15, no. 6, 1 June 2006 (2006-06-01), US, pages 1387 - 1396, XP055346555, ISSN: 0961-8368, DOI: 10.1110/ps.052039606
HUEI-FEN LO ET AL: "Gene Cloning and Biochemical Characterization of a NAD(P)+-Dependent Aldehyde Dehydrogenase from Bacillus licheniformis", MOLECULAR BIOTECHNOLOGY, vol. 46, no. 2, 22 October 2010 (2010-10-22), US, pages 157 - 167, XP055346565, ISSN: 1073-6085, DOI: 10.1007/s12033-010-9290-5
BISCHOFF K M ET AL: "Biosynthesis and characterization of (S)-and (R)-3-hydroxy-3-methylglutaryl coenzyme A", BIOCHEMICAL MEDICINE AND METABOLIC BIOLOGY, ACADEMIC PRESS, vol. 48, no. 2, 1 October 1992 (1992-10-01), pages 149 - 158, XP026273328, ISSN: 0885-4505, [retrieved on 19921001], DOI: 10.1016/0885-4505(92)90060-C
MALEA M. KNEEN ET AL: "Characterization of a thiamin diphosphate-dependent phenylpyruvate decarboxylase from Saccharomyces cerevisiae", FEBS JOURNAL, vol. 278, no. 11, 18 June 2011 (2011-06-18), GB, pages 1842 - 1853, XP055318181, ISSN: 1742-464X, DOI: 10.1111/j.1742-4658.2011.08103.x
JOSÉ SALUD RODRÍGUEZ-ZAVALA ET AL: "Characterization of E. coli tetrameric aldehyde dehydrogenases with atypical properties compared to other aldehyde dehydrogenases", PROTEIN SCIENCE, vol. 15, no. 6, 1 June 2006 (2006-06-01), US, pages 1387 - 1396, XP055346555, ISSN: 0961-8368, DOI: 10.1110/ps.052039606
HUEI-FEN LO ET AL: "Gene Cloning and Biochemical Characterization of a NAD(P)+-Dependent Aldehyde Dehydrogenase from Bacillus licheniformis", MOLECULAR BIOTECHNOLOGY, vol. 46, no. 2, 22 October 2010 (2010-10-22), US, pages 157 - 167, XP055346565, ISSN: 1073-6085, DOI: 10.1007/s12033-010-9290-5
BISCHOFF K M ET AL: "Biosynthesis and characterization of (S)-and (R)-3-hydroxy-3-methylglutaryl coenzyme A", BIOCHEMICAL MEDICINE AND METABOLIC BIOLOGY, ACADEMIC PRESS, vol. 48, no. 2, 1 October 1992 (1992-10-01), pages 149 - 158, XP026273328, ISSN: 0885-4505, [retrieved on 19921001], DOI: 10.1016/0885-4505(92)90060-C
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