Title:
METHODS OF CHARACTERISING CANCER
Document Type and Number:
WIPO Patent Application WO/2016/203053
Kind Code:
A3
Abstract:
The present invention concerns the use of immunoassays for the characterisation, diagnosis, screening and monitoring of cancer and other diseases. In particular said immunoassays involve the use of polyclonal and monoclonal antibodies against different regions in tissue factor, particularly urinary tissue factor, and methods for generating the same. Preferably said antibodies are specific for the tissue factor signal transduction peptide region (anti-TF-STP antibodies). In particular, the present invention relates to antibodies and the use thereof which are specific for phosphorylated isoforms of TF-STP. The invention further concerns use of these anti-TF-STP antibodies to quantitate TF-STP isoformsin biological fluids, in particular urine. Also described herein is the use ofsaid antibodies in immunoassays for the characterisation, diagnosis, screening and monitoring of cancer and other diseases, specificpolyclonal and monoclonal antibodies against different regions in tissue factor, and methods for generating the same.
Inventors:
HERD THOMAS MOLE (GB)
GREENFIELD ROBERT SCOTT (US)
GREENFIELD ROBERT SCOTT (US)
Application Number:
PCT/EP2016/064219
Publication Date:
February 09, 2017
Filing Date:
June 20, 2016
Export Citation:
Assignee:
HERD THOMAS MOLE (GB)
GREENFIELD ROBERT SCOTT (US)
GREENFIELD ROBERT SCOTT (US)
International Classes:
G01N33/68; G01N33/86
Domestic Patent References:
| WO2012052762A1 | 2012-04-26 |
Other References:
UNRUH DUSTEN ET AL: "Levels of Alternatively Spliced Tissue Factor in the Plasma of Patients with Pancreatic Cancer May Help Predict Aggressive Tumor Phenotype", ANNALS OF SURGICAL ONCOLOGY, RAVEN PRESS, NEW YORK, NY, US, vol. 22, no. 3, 12 May 2015 (2015-05-12), pages 1206 - 1211, XP035897259, ISSN: 1068-9265, [retrieved on 20150512], DOI: 10.1245/S10434-015-4592-2
M. E. W. COLLIER ET AL: "Regulation of the Incorporation of Tissue Factor into Microparticles by Serine Phosphorylation of the Cytoplasmic Domain of Tissue Factor", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 286, no. 14, 10 February 2011 (2011-02-10), US, pages 11977 - 11984, XP055312371, ISSN: 0021-9258, DOI: 10.1074/jbc.M110.195214
CAMILLE ETTELAIE ET AL: "p38[alpha] phosphorylates serine 258 within the cytoplasmic domain of tissue factor and prevents its incorporation into cell-derived microparticles", BIOCHIMICA ET BIOPHYSICA ACTA. MOLECULAR CELL RESEARCH., vol. 1833, no. 3, 1 March 2013 (2013-03-01), NL, pages 613 - 621, XP055312370, ISSN: 0167-4889, DOI: 10.1016/j.bbamcr.2012.11.010
A. DORFLEUTNER ET AL: "Regulation of tissue factor cytoplasmic domain phosphorylation by palmitoylation", BLOOD, vol. 102, no. 12, 1 December 2003 (2003-12-01), US, pages 3998 - 4005, XP055312517, ISSN: 0006-4971, DOI: 10.1182/blood-2003-04-1149
RUF W ET AL: "Tissue factor in cancer progression and angiogenesis", THROMBOSIS RESEARCH, TARRYTOWN, NY, US, vol. 125, 1 April 2010 (2010-04-01), pages S36 - S38, XP027032686, ISSN: 0049-3848, [retrieved on 20100401]
LISA RYDÃ N ET AL: "Evidence for tissue factor phosphorylation and its correlation with protease-activated receptor expression and the prognosis of primary breast cancer", INTERNATIONAL JOURNAL OF CANCER, 1 January 2009 (2009-01-01), US, pages NA - NA, XP055313367, ISSN: 0020-7136, DOI: 10.1002/ijc.24921
BELTING MATTIAS ET AL: "Regulation of angiogenesis by tissue factor cytoplasmic domain signaling", NATURE MEDICINE, NATURE PUBLISHING GROUP, NEW YORK, NY, US, vol. 10, no. 5, 1 May 2004 (2004-05-01), pages 502 - 509, XP002488525, ISSN: 1078-8956, [retrieved on 20040418], DOI: 10.1038/NM1037
BEGÜM KOCATÜRK ET AL: "Tissue factor isoforms in cancer and coagulation: May the best isoform win", THROMBOSIS RESEARCH, vol. 129, no. 1, 1 April 2012 (2012-04-01), US, pages S69 - S75, XP055312269, ISSN: 0049-3848, DOI: 10.1016/S0049-3848(12)70020-8
ULRIKE LEPPERT ET AL: "The role of tissue factor isoforms in cancer biology", INTERNATIONAL JOURNAL OF CANCER, vol. 137, no. 3, 16 May 2014 (2014-05-16), US, pages 497 - 503, XP055312268, ISSN: 0020-7136, DOI: 10.1002/ijc.28959
ANDREAS EISENREICH ET AL: "Regulation and Differential Role of the Tissue Factor Isoforms in Cardiovascular Biology", TRENDS IN CARDIOVASCULAR MEDICINE, vol. 20, no. 6, 2010, pages 199 - 203, XP028335427, ISSN: 1050-1738, [retrieved on 20110803], DOI: 10.1016/J.TCM.2011.08.001
M. DAVILA ET AL: "Microparticle association and heterogeneity of tumor-derived tissue factor in plasma: is it important for coagulation activation?", JOURNAL OF THROMBOSIS AND HAEMOSTASIS, vol. 12, no. 2, 1 February 2014 (2014-02-01), GB, pages 186 - 196, XP055312290, ISSN: 1538-7933, DOI: 10.1111/jth.12475
M. E. W. COLLIER ET AL: "Regulation of the Incorporation of Tissue Factor into Microparticles by Serine Phosphorylation of the Cytoplasmic Domain of Tissue Factor", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 286, no. 14, 10 February 2011 (2011-02-10), US, pages 11977 - 11984, XP055312371, ISSN: 0021-9258, DOI: 10.1074/jbc.M110.195214
CAMILLE ETTELAIE ET AL: "p38[alpha] phosphorylates serine 258 within the cytoplasmic domain of tissue factor and prevents its incorporation into cell-derived microparticles", BIOCHIMICA ET BIOPHYSICA ACTA. MOLECULAR CELL RESEARCH., vol. 1833, no. 3, 1 March 2013 (2013-03-01), NL, pages 613 - 621, XP055312370, ISSN: 0167-4889, DOI: 10.1016/j.bbamcr.2012.11.010
A. DORFLEUTNER ET AL: "Regulation of tissue factor cytoplasmic domain phosphorylation by palmitoylation", BLOOD, vol. 102, no. 12, 1 December 2003 (2003-12-01), US, pages 3998 - 4005, XP055312517, ISSN: 0006-4971, DOI: 10.1182/blood-2003-04-1149
RUF W ET AL: "Tissue factor in cancer progression and angiogenesis", THROMBOSIS RESEARCH, TARRYTOWN, NY, US, vol. 125, 1 April 2010 (2010-04-01), pages S36 - S38, XP027032686, ISSN: 0049-3848, [retrieved on 20100401]
LISA RYDÃ N ET AL: "Evidence for tissue factor phosphorylation and its correlation with protease-activated receptor expression and the prognosis of primary breast cancer", INTERNATIONAL JOURNAL OF CANCER, 1 January 2009 (2009-01-01), US, pages NA - NA, XP055313367, ISSN: 0020-7136, DOI: 10.1002/ijc.24921
BELTING MATTIAS ET AL: "Regulation of angiogenesis by tissue factor cytoplasmic domain signaling", NATURE MEDICINE, NATURE PUBLISHING GROUP, NEW YORK, NY, US, vol. 10, no. 5, 1 May 2004 (2004-05-01), pages 502 - 509, XP002488525, ISSN: 1078-8956, [retrieved on 20040418], DOI: 10.1038/NM1037
BEGÜM KOCATÜRK ET AL: "Tissue factor isoforms in cancer and coagulation: May the best isoform win", THROMBOSIS RESEARCH, vol. 129, no. 1, 1 April 2012 (2012-04-01), US, pages S69 - S75, XP055312269, ISSN: 0049-3848, DOI: 10.1016/S0049-3848(12)70020-8
ULRIKE LEPPERT ET AL: "The role of tissue factor isoforms in cancer biology", INTERNATIONAL JOURNAL OF CANCER, vol. 137, no. 3, 16 May 2014 (2014-05-16), US, pages 497 - 503, XP055312268, ISSN: 0020-7136, DOI: 10.1002/ijc.28959
ANDREAS EISENREICH ET AL: "Regulation and Differential Role of the Tissue Factor Isoforms in Cardiovascular Biology", TRENDS IN CARDIOVASCULAR MEDICINE, vol. 20, no. 6, 2010, pages 199 - 203, XP028335427, ISSN: 1050-1738, [retrieved on 20110803], DOI: 10.1016/J.TCM.2011.08.001
M. DAVILA ET AL: "Microparticle association and heterogeneity of tumor-derived tissue factor in plasma: is it important for coagulation activation?", JOURNAL OF THROMBOSIS AND HAEMOSTASIS, vol. 12, no. 2, 1 February 2014 (2014-02-01), GB, pages 186 - 196, XP055312290, ISSN: 1538-7933, DOI: 10.1111/jth.12475
Attorney, Agent or Firm:
BIRTLE, Zoe (GB)
Download PDF: