Title:
METHODS AND COMPOSITIONS FOR DETECTING THE ACTIVATION STATE OF THE MULTIPLE PROTEINS IN SINGLE CELLS
Document Type and Number:
WIPO Patent Application WO2003067210
Kind Code:
A3
Abstract:
The invention provides methods and compositions for simultaneously detecting the activation state of a plurality of proteins in single cells using flow cytometry. The invention further provides methods and compositions of the screening for bioactive agents capable of coordinately modulating the activity of a plurality of proteins in single cells. The methods and compositions can be used to determine the protein activation profile of a cell for predicting or diagnosing a disease state, and for monitoring treatment of a disease state.
More Like This:
Inventors:
PEREZ OMAR D (US)
NOLAN GARRY P (US)
NOLAN GARRY P (US)
Application Number:
PCT/US2002/022328
Publication Date:
December 18, 2003
Filing Date:
July 10, 2002
Export Citation:
Assignee:
UNIV LELAND STANFORD JUNIOR (US)
PEREZ OMAR D (US)
NOLAN GARRY P (US)
PEREZ OMAR D (US)
NOLAN GARRY P (US)
International Classes:
C12Q1/00; C12Q1/02; C12Q1/37; C12Q1/48; G01N21/78; G01N33/50; G01N33/53; G01N33/543; G01N33/569; G01N33/573; (IPC1-7): G01N33/53; G01N33/543
Foreign References:
| US5599681A | 1997-02-04 | |||
| US20020127604A1 | 2002-09-12 |
Other References:
NOLAN ET AL.: "Fluorescence-activated cell analysis and sorting of viable mammalian cells based on B-D-galactosidase activity after transduction of escherichia coli lacZ", PROC. NATL. ACAD. SCI. USA, vol. 85, April 1988 (1988-04-01), pages 2603 - 2607, XP002969136
FIERING ET AL.: "Improved FACS-Gal: flow cytometric analysis and sorting of viable eukaryotic cells expressing reporter gene constructs", CYTOMETRY, vol. 12, 1991, pages 291 - 301, XP000829505
ANDERSON ET AL.: "Simultaneous fluorescence-activated cell sorter analysis of two distinct transcriptional elements with a single cell using engineered green fluorescent proteins", PROC. NATL. ACAD. SCI. USA, vol. 93, August 1996 (1996-08-01), pages 8508 - 8511, XP002189455
FIERING ET AL.: "Improved FACS-Gal: flow cytometric analysis and sorting of viable eukaryotic cells expressing reporter gene constructs", CYTOMETRY, vol. 12, 1991, pages 291 - 301, XP000829505
ANDERSON ET AL.: "Simultaneous fluorescence-activated cell sorter analysis of two distinct transcriptional elements with a single cell using engineered green fluorescent proteins", PROC. NATL. ACAD. SCI. USA, vol. 93, August 1996 (1996-08-01), pages 8508 - 8511, XP002189455
Download PDF: