METHODS AND COMPOSITIONS FOR DETECTING PROMOTER ACTIVITY AND EXPRESSING FUSION PROTEINS

WIPO Patent Application WO2005028615

A3

The present invention provides nucleic acid molecules comprising one or more nucleic acid sequences encoding a polypeptide having a detectable activity. The present invention also provides methods of joining such nucleic acid molecules to nucleic acid molecules to be asseyed for promoter activity. The present invention also relates to methods of preparing fusion proteins comprising a polypeptide of interest and a polypeptyde having a detectable activity.

WELCH PETER J (US)
CHESNUT JONATHAN D (US)
BENNETT ROBERT P (US)
FRIMPONG KENNETH (US)
LEONG LOUIS (US)
FAN JAMES (US)
YIM HARRY (US)
VOZZA-BROWN LAURA (US)

PCT/US2004/020747

August 25, 2005

June 28, 2004

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INVITROGEN CORP (US)
WELCH PETER J (US)
CHESNUT JONATHAN D (US)
BENNETT ROBERT P (US)
FRIMPONG KENNETH (US)
LEONG LOUIS (US)
FAN JAMES (US)
YIM HARRY (US)
VOZZA-BROWN LAURA (US)

C07H21/04; C12N9/22; C12N9/80; C12N15/10; C12N15/64; C12N15/66; C12Q1/68; C12N; (IPC1-7): C12Q1/68; C07H21/04; C12N9/22; C12N9/80

US6468754B1

2002-10-22

"Topo TA Cloning Kits with Gateway Technology", INVITROGEN, 30 May 2005 (2005-05-30), XP002987938, Retrieved from the Internet
VERGUNST ET AL: "Site-specific integraino of Agrobacterium T-DNA in Arabidopsis thaliana mediated by Cre recombinase", NUCLEIC ACID RESEARCH, vol. 26, no. 11, 1998, pages 2729 - 2734, XP002300998

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