Title:
METHODS AND COMPOSITIONS FOR MODIFYING GENOMIC DNA
Document Type and Number:
WIPO Patent Application WO/2018/015936
Kind Code:
A3
Abstract:
Compositions and methods concern the sequence modification of an endogenous genomic DNA region. Certain aspects relate to a method for site-specific sequence modification of a target genomic DNA region in cells comprising: transfecting the cells by electroporation with a composition comprising (a) a DNA oligo; (b) a DNA digesting agent; and (c) a targeting RNA, wherein the targeting RNA is capped and/or polyadenylated; wherein the donor DNA comprises: (i) a homologous region comprising nucleic acid sequence homologous to the target genomic DNA region and (ii) a sequence modification region; and wherein the genomic DNA sequence is modified specifically at the target genomic DNA region.
Inventors:
LI LINHONG (US)
ALLEN CORNELL (US)
PESHWA MADHUSUDAN (US)
ALLEN CORNELL (US)
PESHWA MADHUSUDAN (US)
Application Number:
PCT/IB2017/054446
Publication Date:
March 01, 2018
Filing Date:
July 21, 2017
Export Citation:
Assignee:
MAXCYTE INC (US)
International Classes:
C12N5/10; C12N9/22; C12N15/82; C12N15/90
Domestic Patent References:
| WO2015160683A1 | 2015-10-22 |
Other References:
ZHANG, Y ET AL.: "Biallelic targeting of expressed genes in mouse embryonic stem cells using the Cas9 system", METHODS, vol. 69, no. 2, September 2014 (2014-09-01), pages 171 - 178, XP055242844
HASHIMOTO, M ET AL.: "Electroporation of Cas9 protein/sgRNA into early pronuclear zygotes generates non-mosaic mutants in the mouse", DEVELOPMENTAL BIOLOGY, vol. 418, no. 1, 1 October 2016 (2016-10-01), pages 1 - 9, XP029733963
HASHIMOTO, M ET AL.: "Electroporation of Cas9 protein/sgRNA into early pronuclear zygotes generates non-mosaic mutants in the mouse", DEVELOPMENTAL BIOLOGY, vol. 418, no. 1, 1 October 2016 (2016-10-01), pages 1 - 9, XP029733963
Attorney, Agent or Firm:
STELLMAN, Laurie et al. (US)
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