Title:
METHODS AND COMPOSITIONS FOR PURIFICATION OF NUCLEIC ACID FROM A HOST CELL
Document Type and Number:
WIPO Patent Application WO2004081175
Kind Code:
A3
Abstract:
Methods and compositions are provided for gently lysing and solubilizing cells. Methods and compositions are further provided for quickly purifying high quality low molecular weight nucleic acid from host cells. Target cells are treated with a pre-chilled lysis solution having a zwitterionic detergent, for example n-Dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate, and a brief room-temperature incubation. Where nucleic acid purification is required, the lysis solution-treated cells are contacted with a nucleic acid capture matrix having an average pore size of at least about 1µm.
Inventors:
DOMANICO MICHAEL J
MEYERS MATT
KEANE KRISTIAN
BRAUN LISA R
KOLZAU THOMAS
MEYERS MATT
KEANE KRISTIAN
BRAUN LISA R
KOLZAU THOMAS
Application Number:
PCT/US2003/040175
Publication Date:
February 03, 2005
Filing Date:
December 16, 2003
Export Citation:
Assignee:
EPPENDORF 5 PRIME INC (US)
International Classes:
C07H21/00; C12N1/06; C12N15/10; C12Q1/68; G01N1/18; G01N33/48; C12N; (IPC1-7): G01N33/48; C07H21/00; C12Q1/68
Foreign References:
| US4652517A | 1987-03-24 | |||
| US20020012982A1 | 2002-01-31 | |||
| US20030134412A1 | 2003-07-17 | |||
| US6548256B2 | 2003-04-15 |
Other References:
See also references of EP 1601966A4
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