Title:
METHODS AND COMPOSITIONS RELATING TO ENZYME ASSAYS
Document Type and Number:
WIPO Patent Application WO/2010/083286
Kind Code:
A3
Abstract:
Methods of assaying enzyme-mediated oxidative demethylation are provided according to embodiments of the present invention which includes combining, under reaction conditions, an oxidative demethylation enzyme, a substrate for the oxidative demethylation enzyme and a formaldehyde detection reagent. Detection of fluorescence is indicative of formaldehyde generated by oxidative demethylation of the substrate by the enzyme, the fluorescence resulting from reaction of formaldehyde and the formaldehyde detection reagent.
Inventors:
HART RUSSELL (US)
SCHEUER BARBARA (US)
TRIEVEL RAYMOND (US)
SCHEUER BARBARA (US)
TRIEVEL RAYMOND (US)
Application Number:
PCT/US2010/021008
Publication Date:
February 17, 2011
Filing Date:
January 14, 2010
Export Citation:
Assignee:
LUMINOS LLC (US)
HART RUSSELL (US)
SCHEUER BARBARA (US)
TRIEVEL RAYMOND (US)
HART RUSSELL (US)
SCHEUER BARBARA (US)
TRIEVEL RAYMOND (US)
International Classes:
C12Q1/26; C07C47/04; G01N31/00
Other References:
COUTURE, J. F. ET AL., NATURE STRUCTURAL & MOLECULAR BIOLOGY., vol. 14, no. 8, 24 June 2007 (2007-06-24), pages 689 - 695
OLIVEIRA, F. S. ET AL., TALANTA., vol. 73, 4 May 2007 (2007-05-04), pages 561 - 566
SHI, Y. ET AL., CELL., vol. 119, 29 December 2004 (2004-12-29), pages 941 - 953
TSUKADA, Y. ET AL., NATURE., vol. 439, no. 16, 18 December 2005 (2005-12-18), pages 811 - 816
LI, Q. ET AL., TALANTA., vol. 72, 1 February 2007 (2007-02-01), pages 1675 - 1680
CHANG, B. ET AL., SCIENCE., vol. 318, 19 October 2007 (2007-10-19), pages 444 - 447
OLIVEIRA, F. S. ET AL., TALANTA., vol. 73, 4 May 2007 (2007-05-04), pages 561 - 566
SHI, Y. ET AL., CELL., vol. 119, 29 December 2004 (2004-12-29), pages 941 - 953
TSUKADA, Y. ET AL., NATURE., vol. 439, no. 16, 18 December 2005 (2005-12-18), pages 811 - 816
LI, Q. ET AL., TALANTA., vol. 72, 1 February 2007 (2007-02-01), pages 1675 - 1680
CHANG, B. ET AL., SCIENCE., vol. 318, 19 October 2007 (2007-10-19), pages 444 - 447
Attorney, Agent or Firm:
STAPLE, Julie, K. et al. (Krass Sprinkle, Anderson & Citkowski, P.C.,2701 Troy Center Drive, Suite 330, PO Box 702, Troy MI, US)
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