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Patent Searching and Data


Title:
METHODS AND COMPOSITIONS FOR RISK STRATIFICATION
Document Type and Number:
WIPO Patent Application WO2006012507
Kind Code:
A3
Abstract:
The present invention provides an approach for the simultaneous determination of the activation states of a plurality of proteins in single cells. This approach permits the rapid detection of heterogeneity in a complex cell population based on activation states, and the identification of cellular subsets that exhibit correlated changes in activation within the cell population. Moreover, this approach allows the correlation of cellular activities or properties. In addition, the use of potentiators of cellular activation allows for characterization of such pathways and cell populations.

Inventors:
PEREZ OMAR D (US)
NOLAN GARRY P (US)
IRISH JONATHAN M (US)
Application Number:
PCT/US2005/026026
Publication Date:
October 12, 2006
Filing Date:
July 21, 2005
Export Citation:
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Assignee:
UNIV LELAND STANFORD JUNIOR (US)
PEREZ OMAR D (US)
NOLAN GARRY P (US)
IRISH JONATHAN M (US)
International Classes:
G01N33/50; C12Q1/48; G01N15/14; G01N33/543; G01N33/569; G01N33/573
Domestic Patent References:
WO2003067210A22003-08-14
Foreign References:
US5968738A1999-10-19
Other References:
PEREZ O.D. ET AL.: "Simultaneous measurement of multiple active kinase states using polychromatic flow cytometry.", NATURE BIOTECH., vol. 20, February 2002 (2002-02-01), pages 155 - 162, XP002386690
KRUTZIK P.O. ET AL.: "Analysis of protein phosphorylation and cellular signaling events by flow cytometry: techniques and clinical applications.", CLIN. IMMUNOL., vol. 110, March 2004 (2004-03-01), pages 206 - 221, XP002386691
IRISH J.M. ET AL.: "Single cell profiling of potentiated phospho-protein networks in cancer cells.", CELL, vol. 118, 23 July 2004 (2004-07-23), pages 217 - 228, XP002386692
PEREZ O.D. ET AL.: "LFA-1 signaling through p44/42 is coupled to perforin degranulation in CD56+CD8+ natural killer cells.", BLOOD, vol. 104, no. 4, 15 August 2004 (2004-08-15), pages 1083 - 1093, XP002386693
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