Title:
METHODS AND COMPOSITIONS FOR RNA INTERFERENCE
Document Type and Number:
WIPO Patent Application WO2005018534
Kind Code:
A3
Abstract:
The invention provides methods and compositions for gene silencing by RNA interference. In particular, the invention provides methods for gene silencing or RNA knockdown using small interfering RNAs (siRNAs) having partial sequence homology to its target gene. The invention also provides methods for identifying common and/or differential responses to a plurality of different siRNAs targeting a gene. The invention also provides methods for evaluating the relative activity of the two strands of an siRNA. The invention further provides methods of designing siRNAs for gene silencing. The invention further provides methods of using siRNAs as therapeutics for treatment of diseases.
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Inventors:
JACKSON AIMEE L (US)
BARTZ STEVEN R (US)
BURCHARD JULIA (US)
SCHELTER JANELL M (US)
LINSLEY PETER S (US)
BARTZ STEVEN R (US)
BURCHARD JULIA (US)
SCHELTER JANELL M (US)
LINSLEY PETER S (US)
Application Number:
PCT/US2004/015439
Publication Date:
December 08, 2005
Filing Date:
May 17, 2004
Export Citation:
Assignee:
ROSETTA INPHARMATICS LLC (US)
JACKSON AIMEE L (US)
BARTZ STEVEN R (US)
BURCHARD JULIA (US)
SCHELTER JANELL M (US)
LINSLEY PETER S (US)
JACKSON AIMEE L (US)
BARTZ STEVEN R (US)
BURCHARD JULIA (US)
SCHELTER JANELL M (US)
LINSLEY PETER S (US)
International Classes:
A61K48/00; C07H21/04; C12N15/113; A61K; (IPC1-7): C07H21/04; A61K48/00
Domestic Patent References:
| WO2002044321A2 | 2002-06-06 |
Other References:
GREEN D.W. ET AL: "Antisense Oligonucleotides: An evolving technology for the modulation for gene expression in human disease.", J AM COLL SURG., vol. 191, 2000, pages 93 - 105, XP002952060
JEN K.Y. ET AL: "Suppresion of gene expression by targeted disruption of messenger RNA: available options and current strategies.", STEM CELLS., vol. 18, 2000, pages 307 - 318, XP002181426
CAPLEN N.J. ET AL: "RNAi is a gene therapy approach.", GENE THERAPY., vol. 3, no. 4, 2003, pages 575 - 586, XP008055014
PAROO Z. ET AL: "Challenges for RNAi in vivo.", TRENDS IN BIOTECHNOLOGY., vol. 22, 2004, pages 309 - 394, XP002299924
NOVINA C.D. ET AL: "The RNAi revolution.", NATURE., vol. 430, 2004, pages 161 - 164, XP002978290
JEN K.Y. ET AL: "Suppresion of gene expression by targeted disruption of messenger RNA: available options and current strategies.", STEM CELLS., vol. 18, 2000, pages 307 - 318, XP002181426
CAPLEN N.J. ET AL: "RNAi is a gene therapy approach.", GENE THERAPY., vol. 3, no. 4, 2003, pages 575 - 586, XP008055014
PAROO Z. ET AL: "Challenges for RNAi in vivo.", TRENDS IN BIOTECHNOLOGY., vol. 22, 2004, pages 309 - 394, XP002299924
NOVINA C.D. ET AL: "The RNAi revolution.", NATURE., vol. 430, 2004, pages 161 - 164, XP002978290
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