Title:
METHODS AND COMPOSITIONS FOR SINGLE CHAIN VARIABLE REGION ENOX2 ANTIBODIES FOR CANCER DETECTION AND DIAGNOSIS
Document Type and Number:
WIPO Patent Application WO/2013/052926
Kind Code:
A3
Abstract:
[0076] All neoplastic cells express one or more members of a unique family of cell surface ubiquinone (NADH) oxidase proteins with protein disulfide-thiol interchange activity (ECTO-NOX proteins) that are characteristically inhibited by quinone site inhibitors with anti-cancer activity and a common amino acid sequence disclosed herein that allows for cancer-specific antibody recognition as well as for detection of certain critical reference proteins that provide loading controls. Cancers of different cellular or tissue origins express different ENOX2 cancer isoforms or combinations of isoforms and shed these proteins into the circulation. Herein are disclosed methods both for cancer detection and diagnosis of particular origin, based on the patterns and molecular weights of the isoforms which allow the identification of the cell type and or tissue of origin of the neoplasm. Relative ENOX2 amounts are proportional to tumor burden and provide a reliable measure of response to therapy and disease progression. Also provided is the amino acid sequence to which the scFv antibodies bind as the molecular basis for the specificity of the test.
Inventors:
MORRE D JAMES (US)
HOSTETLER BRANDON EUGENE (US)
KIM JAMES JINPAL (US)
HOSTETLER BRANDON EUGENE (US)
KIM JAMES JINPAL (US)
Application Number:
PCT/US2012/059141
Publication Date:
May 30, 2013
Filing Date:
October 05, 2012
Export Citation:
Assignee:
MOR NUTECH INC (US)
International Classes:
G01N33/53; C07K16/00; G01N33/52; G01N33/532; G01N33/574
Domestic Patent References:
| WO2011109663A1 | 2011-09-09 |
Foreign References:
| US20090042209A1 | 2009-02-12 |
Other References:
MORRE ET AL.: "Cancer type-specific tNOX isoform: A putative family of redox protein splice variants with cancer diagnostic and prognostic potential", BIOFACTORS, vol. 34, no. 3, 2008, pages 201 - 207, XP055070359
MORRE ET AL.: "Early detection : An opportunity for cancer prevention through early intervention", CANCER PREVENTION - FROM MECHANISMS TO TRANSLATIONAL BENEFITS, 20 April 2012 (2012-04-20), pages 389 - 402
MORRE ET AL.: "Early detection : An opportunity for cancer prevention through early intervention", CANCER PREVENTION - FROM MECHANISMS TO TRANSLATIONAL BENEFITS, 20 April 2012 (2012-04-20), pages 389 - 402
Attorney, Agent or Firm:
MCNETT, John, C. et al. (EMHARDT MORIARTY, MCNETT & HENRY LLP,111 Monument Circle, Suite 370, Indianapolis IN, US)
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