Title:
METHODS AND COMPOSITIONS FOR USE IN HOMOLOGOUS RECOMBINATION IN PLANT CELLS
Document Type and Number:
WIPO Patent Application WO2004106496
Kind Code:
A3
Abstract:
Methods for homologously recombining an exogenous nucleic acid into a plant target cell genome of a plant target cell are provided. In the subject methods, a targeting vector that includes a linearizing endonuclease site, e.g., a recombinase recognition site, and a homologous recombination integrating element, is contacted with the plant target cell(s) such that the plant target cell(s) take up the targeting vector. The targeting vector is originally a circular targeting vector that is linearized by a linearizing endonuclease, e.g., a recombinase, either prior to administration (e.g., in vitro treatment with an endonuclease) or upon entry into the multicellular organism by endonucleases in the extracellular or intracellular fluids that can be introduced to or already present in the muticellular organism. The integrating element homologously recombines into the plant target cell genome from the linearized targeting vector. Also provided are targeting vectors, systems and kits for use in practicing the subject methods.
Inventors:
FOGARTY PATRICK (US)
Application Number:
PCT/US2004/016798
Publication Date:
June 09, 2005
Filing Date:
May 26, 2004
Export Citation:
Assignee:
TOSK INC (US)
FOGARTY PATRICK (US)
FOGARTY PATRICK (US)
International Classes:
A01H1/00; C12N15/11; C12N15/82; C12N15/87; C12N; (IPC1-7): C12N15/87; A01H1/00; C12N15/11; C12N15/82
Other References:
PUCHTA ET AL: "Two different but related mechanisms are used in plants for the repair of genomic double-strand breaks by homologous recombination", PNAS, vol. 93, 1996, pages 5055 - 5060, XP002236852
PUCHTA ET AL: "HOMOLOGOUS RECOMBINATION IN PLANT CELLS IS ENHANCED BY IN VIVO INDUCTION OF DOUBLE STRAND BREAKS INTO DNA BY A SITE-SPECIFIC ENDONUCLEASE", NUCLEID ACIDS RESEARCH, vol. 21, no. 22, 1993, pages 5034 - 5040, XP000571587
GISLER ET AL: "The Role of Double-Stranded Break-Induced Allelic Homologous Recombination in Somatic Plant Cells", THE PLANT JOURNAL, vol. 32, 2002, pages 277 - 284
PUCHTA ET AL: "HOMOLOGOUS RECOMBINATION IN PLANT CELLS IS ENHANCED BY IN VIVO INDUCTION OF DOUBLE STRAND BREAKS INTO DNA BY A SITE-SPECIFIC ENDONUCLEASE", NUCLEID ACIDS RESEARCH, vol. 21, no. 22, 1993, pages 5034 - 5040, XP000571587
GISLER ET AL: "The Role of Double-Stranded Break-Induced Allelic Homologous Recombination in Somatic Plant Cells", THE PLANT JOURNAL, vol. 32, 2002, pages 277 - 284
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