Title:
METHODS FOR CONTROLLING GIBBERELLIN LEVELS
Document Type and Number:
WIPO Patent Application WO2000009722
Kind Code:
A3
Abstract:
Methods and materials are disclosed for the inhibition and control of gibberellic acid levels. In particular, nucleic acid sequences of copalyl diphosphate synthase, 3- beta hydroxylase, and 2-oxidase and additional nucleic acid sequences are disclosed. Gibberellic acid levels may be inhibited or controlled by preparation of a chimeric expression construct capable of expressing a RNA or protein product which suppresses the gibberellin biosynthetic pathway sequence, diverts substrates from the pathway or degrades pathway substrates or products. The sequence is preferably a copalyl diphosphate synthase sequence, a 3 beta -hydroxylase sequence, a 2-oxidase sequence, a phytoene synthase sequence, a C20-oxidase sequence, and a 2 beta ,3 beta -hydroxylase sequence. Administration of a complementing agent, preferably a gibberellin or gibberellin precursor or intermediate restores bioactivity.
Inventors:
BROWN SHERRI M (US)
ELICH TEDD D (US)
HECK GREGORY R (US)
KISHORE GANESH M (US)
LOGUSCH EUGENE W (US)
LOGUSCH SHERRY J (US)
PILLER KENNETH J (US)
RAO SUDABATHULA (US)
REAM JOEL E (US)
ELICH TEDD D (US)
HECK GREGORY R (US)
KISHORE GANESH M (US)
LOGUSCH EUGENE W (US)
LOGUSCH SHERRY J (US)
PILLER KENNETH J (US)
RAO SUDABATHULA (US)
REAM JOEL E (US)
Application Number:
PCT/US1999/018066
Publication Date:
September 28, 2000
Filing Date:
August 10, 1999
Export Citation:
Assignee:
MONSANTO CO (US)
BROWN SHERRI M (US)
ELICH TEDD D (US)
HECK GREGORY R (US)
KISHORE GANESH M (US)
LOGUSCH EUGENE W (US)
LOGUSCH SHERRY J (US)
PILLER KENNETH J (US)
RAO SUDABATHULA (US)
REAM JOEL E (US)
BROWN SHERRI M (US)
ELICH TEDD D (US)
HECK GREGORY R (US)
KISHORE GANESH M (US)
LOGUSCH EUGENE W (US)
LOGUSCH SHERRY J (US)
PILLER KENNETH J (US)
RAO SUDABATHULA (US)
REAM JOEL E (US)
International Classes:
C12N9/00; C12N9/02; C12N15/53; C12N15/82; (IPC1-7): C12N15/82; C12N15/53; C12N15/54; C12N9/02; C12N9/10; C12N9/00; A01H5/00
Domestic Patent References:
| WO1997029123A2 | 1997-08-14 | |||
| WO1997043419A2 | 1997-11-20 | |||
| WO1993016096A1 | 1993-08-19 | |||
| WO1995035383A2 | 1995-12-28 | |||
| WO1994028141A1 | 1994-12-08 | |||
| WO1995002060A1 | 1995-01-19 | |||
| WO1996005317A1 | 1996-02-22 | |||
| WO1997046690A1 | 1997-12-11 | |||
| WO1997041240A1 | 1997-11-06 | |||
| WO1999066029A2 | 1999-12-23 |
Foreign References:
| EP0692537A2 | 1996-01-17 |
Other References:
FRAY R G ET AL: "CONSTITUTIVE EXPRESSION OF A FRUIT PHYTOENE SYNTHASE GENE IN TRANSGENIC TOMATOES CAUSES DWARFISM BY REDIRECTING METABOLITES FROM THE GIBBERELLIN PATHWAY", PLANT JOURNAL, (NOV 1995) VOL. 8, NO. 5, PP. 693-701. ISSN: 0960-7412., XP002043131
AIT-ALI T. ET AL.: "The LS locus of pea encodes the gibberelin biosynthsis enzyme ent-kaurene synthase A", PLANT JOURNAL, vol. 11, no. 3, 1997, pages 443 - 454, XP002132020
REDDY E.: "AC Y17556", EBI DATABASE, 17 June 1998 (1998-06-17), XP002132021
BENSEN R. ET AL.: "Cloning and chracterization of the maize AN1 gene", PLANT CELL, vol. 7, 1995, pages 75 - 84, XP002040854
WAYCOTT W. AND TAIZ L.: "Phenotypic characterization of lettuce dwarf mutants and their response to applied gibberellins", PLANT PHYSIOLOGY, vol. 95, 1990, pages 1162 - 1168, XP002132022
KUSUBA S. ET AL.: "Decreased GA1 content caused by the overexpression of OSH1 is accompanied by suppression of GA 20-oxidase gene expression", PLANT PHYSIOLOGY, vol. 117, August 1998 (1998-08-01), pages 1179 - 1184, XP002132023
LESTER D. ET AL.: "AC U85045", EBI DATABASE, 22 August 1997 (1997-08-22), XP002139675
LESTER D. ET AL.: "Mendel's stem length gene (Le) encodes a gibberellin 3beta-hydroxylase", PLANT CELL, vol. 9, 1997, pages 1435 - 1443, XP002139676
MACMILLAN J. ET AL.: "Gibberellin biosynthesis from gibberellin a12-aldehyde in endosperm and embryos of Marah macrocarpus", PLANT PHYSIOLOGY, vol. 113, 1997, pages 1369 - 1377, XP002139677
LIN X. ET AL.: "Arabidopsis chromosome II section, AC AC004077", EBI DATABASE, 3 February 1998 (1998-02-03), XP002139678
TOYOMASU T. ET AL.: "rice gibberellin C20-oxidase cDNA, AC U50333", EBI DATABASE, 6 March 1997 (1997-03-06), XP002139679
LANGE T. ET AL.: "Cloning and expression of a gibberellin 2beta,3beta-hydroxylase cDNA from pumpkin endosperm", PLANT CELL, vol. 9, 1997, pages 1459 - 1467, XP002139680
HONG J. ET AL.: "Characterization of a proline-rich cell wall protein gene family of soybean", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 265, no. 5, 1990, pages 2470 - 2475, XP002139681
NAKAMURA Y.: "Arabidopsis genomic clone AC AP000384", EBI DATABASE, 3 August 1999 (1999-08-03), XP002139682
AIT-ALI T. ET AL.: "The LS locus of pea encodes the gibberelin biosynthsis enzyme ent-kaurene synthase A", PLANT JOURNAL, vol. 11, no. 3, 1997, pages 443 - 454, XP002132020
REDDY E.: "AC Y17556", EBI DATABASE, 17 June 1998 (1998-06-17), XP002132021
BENSEN R. ET AL.: "Cloning and chracterization of the maize AN1 gene", PLANT CELL, vol. 7, 1995, pages 75 - 84, XP002040854
WAYCOTT W. AND TAIZ L.: "Phenotypic characterization of lettuce dwarf mutants and their response to applied gibberellins", PLANT PHYSIOLOGY, vol. 95, 1990, pages 1162 - 1168, XP002132022
KUSUBA S. ET AL.: "Decreased GA1 content caused by the overexpression of OSH1 is accompanied by suppression of GA 20-oxidase gene expression", PLANT PHYSIOLOGY, vol. 117, August 1998 (1998-08-01), pages 1179 - 1184, XP002132023
LESTER D. ET AL.: "AC U85045", EBI DATABASE, 22 August 1997 (1997-08-22), XP002139675
LESTER D. ET AL.: "Mendel's stem length gene (Le) encodes a gibberellin 3beta-hydroxylase", PLANT CELL, vol. 9, 1997, pages 1435 - 1443, XP002139676
MACMILLAN J. ET AL.: "Gibberellin biosynthesis from gibberellin a12-aldehyde in endosperm and embryos of Marah macrocarpus", PLANT PHYSIOLOGY, vol. 113, 1997, pages 1369 - 1377, XP002139677
LIN X. ET AL.: "Arabidopsis chromosome II section, AC AC004077", EBI DATABASE, 3 February 1998 (1998-02-03), XP002139678
TOYOMASU T. ET AL.: "rice gibberellin C20-oxidase cDNA, AC U50333", EBI DATABASE, 6 March 1997 (1997-03-06), XP002139679
LANGE T. ET AL.: "Cloning and expression of a gibberellin 2beta,3beta-hydroxylase cDNA from pumpkin endosperm", PLANT CELL, vol. 9, 1997, pages 1459 - 1467, XP002139680
HONG J. ET AL.: "Characterization of a proline-rich cell wall protein gene family of soybean", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 265, no. 5, 1990, pages 2470 - 2475, XP002139681
NAKAMURA Y.: "Arabidopsis genomic clone AC AP000384", EBI DATABASE, 3 August 1999 (1999-08-03), XP002139682
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